What do these following terms mean? Which basis of criteria have they been distinguished?
As Edwin said,
Hypothetical proteins are predicted by gene identification tools during the genome analysis, but for which there is no experimental evidence. When bioinformatic tools were used to identify new genes, it finds the ORF in the genome which do not have an protein analog in the protein database. These proteins show less identity to known, annotated protein
Putative proteins are identified in similar manner but they share sequence similarity to an extent with characterized proteins. Usually, the similarity will be only in their conserved amino acid residues and no other siginificant region will be similar to annotated proteins.
Unknown proteins are predicted and experimentally proven to exist but are not biochemically characterized or they cannot be linked to a known gene.
Take a look at the article below, for more details.
Searching for hypothetical proteins: Theory and practice based
upon original data and literature. Progress in Neurobiology 77 (2005) 90–127
Please check out this paper: about protein annotation in Swiss-Prot:: http://bioinformatics.oxfordjournals.org/content/17/10/920.long and especially the references on page 923.
As Edwin said,
Hypothetical proteins are predicted by gene identification tools during the genome analysis, but for which there is no experimental evidence. When bioinformatic tools were used to identify new genes, it finds the ORF in the genome which do not have an protein analog in the protein database. These proteins show less identity to known, annotated protein
Putative proteins are identified in similar manner but they share sequence similarity to an extent with characterized proteins. Usually, the similarity will be only in their conserved amino acid residues and no other siginificant region will be similar to annotated proteins.
Unknown proteins are predicted and experimentally proven to exist but are not biochemically characterized or they cannot be linked to a known gene.
Take a look at the article below, for more details.
Searching for hypothetical proteins: Theory and practice based
upon original data and literature. Progress in Neurobiology 77 (2005) 90–127
Hypothetical protein: is a protein whose existence has been predicted but for that protein there is no experimental evidence
Putative protein :identified by Bioinformatics tools and servers.
Unknown Protein: those are real cDNA and proteins but they have not been submitted to Genbank.
Hello there
and what if you find such protein in a gel (so you know it exists) but it returns ''hypotetical'' - then it is just a not found homologue, right?
Please provide link for full paper of the below:-
Theory and practice based
upon original data and literature. Progress in Neurobiology 77 (2005) 90–127
Hi Mahanthi, the full title is 'Searching for hypothetical proteins: Theory and practice based upon original data and literature' Its link is
http://www.sciencedirect.com/science/article/pii/S0301008205001152
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