PCR product with smear?

Today I carried out the nested PCR of plasmodium gene which is of 1023 bp and I am not getting proper gel bands, I am getting streaking with my desired band (1023 bp). I have diluted 1:10 times...

09 October 2013 1,971 8 View

Pathogen drug target proteins and their similarity with humans

10 proteins have been predicted as probable drug targets in mycobacterium tuberculosis, while out of 10, only 2 proteins show 20% to 30% similarity with homosapiens. Can we put it into a drug...

02 March 2013 6,784 9 View

Research study

I am beginner in this research field. Can anyone please give me information about: How to Begin Writing a Research Paper for publication in well known Journals.

11 December 2012 5,166 4 View

What are the difference between hypothetical protein, putative protein, and unknown protein?

What do these following terms mean? Which basis of criteria have they been distinguished?

09 October 2012 1,769 7 View

How can a phylogenetic tree produced by the MEGA Software have negative branch lengths?

I have done phylogenetic reconstruction of 50 gens; different length of sequences and at some of the branches I have got a negative branch length. Is that a software error I'm causing or how...

07 August 2012 6,725 14 View

Tools and software for the prediction of percentage of homology among sequences

Could anybody please let me know of offline, or online tools and software for the prediction of % of Homology prediction among 100 nucleotide sequences other than blast?

06 July 2012 5,324 19 View

Insilico Identification of Nucleotide sequence variants of gene

I have a set of 400 nucleotide sequences of plasmodium falciparum gene. Within this set I have to find out the variant nucleotide sequence of that particular gene. If it is a small number if...

06 July 2012 6,904 9 View

Modelling of sequenced gene product

I have sequenced an important part of a variant of the DHPS gene containing 500bp encoding the important domain part of protein, but not the full gene, by Applied Biosystem 16 capillary. Now I...

04 May 2012 6,500 4 View

Branch length

Could anyone please let me know that in phylogenetic analysis how branch length would be in decimal notation ? Branch lengths are usually expressed in units of substitutions per site of the...

02 March 2012 5,579 1 View

Multiple sequence Alignment

could any one plz suggest me tool or software for Multiple sequence alignment Giving Best results (Offline or online ) any ,actually i want to have MSA of 500 Nucleotide sequences giving...

02 March 2012 319 36 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View