PCR product with smear?

Today I carried out the nested PCR of plasmodium gene which is of 1023 bp and I am not getting proper gel bands, I am getting streaking with my desired band (1023 bp). I have diluted 1:10 times...

09 October 2013 1,971 8 View

Pathogen drug target proteins and their similarity with humans

10 proteins have been predicted as probable drug targets in mycobacterium tuberculosis, while out of 10, only 2 proteins show 20% to 30% similarity with homosapiens. Can we put it into a drug...

02 March 2013 6,784 9 View

What are the difference between hypothetical protein, putative protein, and unknown protein?

What do these following terms mean? Which basis of criteria have they been distinguished?

09 October 2012 1,769 7 View

How can a phylogenetic tree produced by the MEGA Software have negative branch lengths?

I have done phylogenetic reconstruction of 50 gens; different length of sequences and at some of the branches I have got a negative branch length. Is that a software error I'm causing or how...

07 August 2012 6,725 14 View

Tools and software for the prediction of percentage of homology among sequences

Could anybody please let me know of offline, or online tools and software for the prediction of % of Homology prediction among 100 nucleotide sequences other than blast?

06 July 2012 5,324 19 View

Insilico Identification of Nucleotide sequence variants of gene

I have a set of 400 nucleotide sequences of plasmodium falciparum gene. Within this set I have to find out the variant nucleotide sequence of that particular gene. If it is a small number if...

06 July 2012 6,904 9 View

Modelling of sequenced gene product

I have sequenced an important part of a variant of the DHPS gene containing 500bp encoding the important domain part of protein, but not the full gene, by Applied Biosystem 16 capillary. Now I...

04 May 2012 6,500 4 View

Branch length

Could anyone please let me know that in phylogenetic analysis how branch length would be in decimal notation ? Branch lengths are usually expressed in units of substitutions per site of the...

02 March 2012 5,579 1 View

Multiple sequence Alignment

could any one plz suggest me tool or software for Multiple sequence alignment Giving Best results (Offline or online ) any ,actually i want to have MSA of 500 Nucleotide sequences giving...

02 March 2012 319 36 View

Biodatabase Framing

Hi, i am working on Msp(merozoite surface protein ) gens of plasmodium species and have downloaded 3000 sequences from different countries original isolate Continental wise of Msp1 to msp 9 , so i...

31 December 2011 6,155 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

No title

01 March 2021 3,905 5 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

Can I follow Researchers from Teaching and testing areas?

I want Researchers from Educational Measurement and Evaluation, relating to teaching, learning , academic performance and test validation

18 February 2021 4,757 13 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Which is the appropriate statistic test to examine the relation between students' affective state and academic performance?

Which test should I use to compare students' mood effects on academic performance? I want to compare mood measured on a likert scale (from point 1= very sad to point= 5 very happy) to three...

04 February 2021 1,907 5 View

How to measure the relationship between students' readiness to E-learning and their academic performance?

I'm currently studying the relationship between students' readiness to E-learning and their academic performance. The variables are: Students' readiness: I intend to measure it through...

03 February 2021 9,207 8 View