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Dear colleagues, i am trying to purify a protein with 26 kDa size that is expressed as a GST fusion protein. When i have done on column GST cleavage in the flow through both GST and my protein of...
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I have cloned a gene with N terminally fused GST in pGEX6P1. II expressed it in BL21(DE3) pLysS cells induced at 0.6 OD 600 with 1mM IPTG, 16 degree celcius overnight in 4 ml of 2YT medium...
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I have cloned a gene in pGEX5X1 with N-terminal GST, C-terminal Flag tag ( total length of protein is 750 amino acid excluding tags ) that is fully unstructured in c-terminal half, and comprising...
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