How can we isolate, characterise and maintain cancer stem cells from lung cancer cells lines?

From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?

02 March 2018 3,131 7 View

How much old protein lysates can be used for western blotting?

Stored at -20 or -80 degrees.

09 October 2016 7,198 3 View

Debris in cell culture ?

I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...

09 October 2016 6,224 10 View

There are different ROS which are produced in cultured cells, DCFDA and DHE both stains and detect these ROS, What are the differences?

How DHE and DCFDA detects ROS differently?

09 October 2016 4,498 4 View

How the concentration of CO2 may effect the growth of cultured cells?

How important Co2 concentration is for cultured cells

09 October 2016 3,274 1 View

What are other antibiotic solutions available for cell culture apart from Streptomycin/Penicillin?

09 October 2016 8,458 3 View

Can we use Caco 2 cells as normal cells for toxicity testing of a chemical?

We don not have normal cells, can we use Caco 2 cells instead

08 September 2016 2,886 4 View

In which cancer, conventional chemotherapy is least beneficial?

With respect to Organs

08 September 2016 8,001 0 View

How can I induce inflammation in gastric cancer cells using LPS?

Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?

05 June 2015 1,451 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Is it possible to quantify recombinant protein in the supernatant using SDS PAGE ?

I would like to quantify a recombinant protein (with native signal sequence) secreted by Pichia pastoris using the SDS PAGE. Since my standard protein (plant origin) is not pure, I would like to...

07 February 2021 3,880 9 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to prepare protein samples in EMSA assay?

Recently l was studying EMSA assay, but l didn't find any detailed information about protein sample. we can use two kinds of protein, including crude protein extracts and recombinant proteins. but...

02 February 2021 10,047 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Alpha synuclein lack structurw (i.e some of the helix part goes into turn ), any cause behind this?

I am trying to simulate alpha synuclein (1xq8.pdb) in GROMACS using GROMOS53a6 ff. I have run it for 200 ns. At the end of simulation alpha helix protein losses its helical structure. I am not...

31 January 2021 4,285 3 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View