How can we isolate, characterise and maintain cancer stem cells from lung cancer cells lines?

From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?

02 March 2018 3,131 7 View

How much old protein lysates can be used for western blotting?

Stored at -20 or -80 degrees.

09 October 2016 7,198 3 View

Debris in cell culture ?

I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...

09 October 2016 6,224 10 View

There are different ROS which are produced in cultured cells, DCFDA and DHE both stains and detect these ROS, What are the differences?

How DHE and DCFDA detects ROS differently?

09 October 2016 4,498 4 View

How the concentration of CO2 may effect the growth of cultured cells?

How important Co2 concentration is for cultured cells

09 October 2016 3,274 1 View

What are other antibiotic solutions available for cell culture apart from Streptomycin/Penicillin?

09 October 2016 8,458 3 View

In which cancer, conventional chemotherapy is least beneficial?

With respect to Organs

08 September 2016 8,001 0 View

What are the best time points to study epigenetic regulation?

For proteins such as HDACs and DNMSts

06 July 2016 9,477 2 View

How can I induce inflammation in gastric cancer cells using LPS?

Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?

05 June 2015 1,451 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View