We don not have normal cells, can we use Caco 2 cells instead
Hi Akash,
You can actually use Caco 2 cells to test a chemical because those cells are normally used in pharmacoceutical industry to predict absorption of drugs, so I think that a toxicity test of a chemical with them would not be a problem.
Thanks Ylenia Roger Valle
Could you suggest any article?
Thanks Udomsak
From A549 and H1299 cells, After isolation which media can be used to maintain cancer stem cells?
02 March 2018 3,257 7 View
Stored at -20 or -80 degrees.
09 October 2016 7,335 3 View
I am observing black dots or debris kind of stuff into culture dishes, fresh media, fresh cell revived but the problem persists. This does not make the media turbid but. This debris is less on the...
09 October 2016 6,384 10 View
How DHE and DCFDA detects ROS differently?
09 October 2016 4,697 4 View
How important Co2 concentration is for cultured cells
09 October 2016 3,401 1 View
What are other antibiotics used and available commercially for animal cell culture
09 October 2016 8,580 3 View
With respect to Organs
08 September 2016 8,121 0 View
For proteins such as HDACs and DNMSts
06 July 2016 9,613 2 View
Please tell which LPS (strain of E.coli) would give best results. Time of induction? what about starvation condition ? I tried with 100ng/ml to 4 ug/ml but could'nt get COX 2 or cCaspase 1?
05 June 2015 1,607 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View