12 January 2016 3 10K Report

I'm looking for literature that details optimal conditions for phage production. 

More Paige Spencer's questions See All
Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What would cause different shape DMA loss modulus curve in different batches of PC+ABS?

I have some DMA curves of PC+ABS from different batches. The PC loss modulus peak is fairly consistent but one batch has a peak value 10degC lower than the rest. Another peaks at the normal value,...

06 December 2020 809 2 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

Phosphomimetic Peptides & Phospho-Specific Antibodies?

I am performing experiments using synthetic cell-penetrating peptides in acute hippocampal slices. Our lab has verified the NLS-mediated nuclear delivery of a number of such peptides in the region...

27 December 2019 7,914 2 View

What is the recommended fixation time of 400 um hippocampal slices in 4% PFA?

I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...

22 June 2018 9,560 5 View

How long does Apex Taq RED Master Mix, 2X remain viable after opening?

The manufacturer's data sheet says the unopened vial lasts for 2 years at -20 C and that the mix is stable at 4 C for at least six months. The dye we are using has been opened and has been kept at...

10 June 2018 3,902 3 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

09 June 2018 9,562 3 View

Is it normal for signal intensity to fluctuate during chemiluminescent detection with Versadoc?

I've noticed this strange phenomenon where my signal intensity will change from picture to picture over the course of my exposure. For example: I will expose membrane to ECL for 2 min, signal...

13 February 2018 1,529 2 View

How to use McNemar's test to compare accuracy of classifications?

Hi, I have seen various studies use McNemar's test to statistically test the accuracy of several classifications. I have six classifications, produced using three different classifiers (SAM, SVM,...

19 September 2017 5,034 4 View

What is the duration of kanamycin selection pressure in common KanR-expressing E. coli LB cultures?

I have used Kanamycin resistance as a selection marker with kanr-containing pET vectors. Since KanR is inactivating the antibiotic (only in the cytoplasm), I was wondering about the duration of...

15 April 2015 8,463 3 View

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What recipe of homemade lenti-virus concentrator do you use?

My lab is using a lenti-X concentrator. And we are looking for a homemade recipe. I have tried this recipe....

08 February 2021 5,965 1 View

How can you adjust pH of SM buffer?

Good afternoon, please how can you adjust pH of phage SM buffer? I presume that main ingredient that has influence of pH is Tris/HCl buffer, but according to literature its range of pH is from 7...

27 January 2021 5,767 3 View

What is the composition of TM buffer used for concentrating bacteriophage?

Hello everyone, I have to isolate DNA from Brucella phage , but before that, I have to concentrate the phage using PEG 8000. I have got protocol for concentrating the phage using PEG and TM buffer...

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How can I transform 50 kb plasmid into ecoli?

I must put 50 kb plasmid into ecoli strain. I think 50 kb is too big for heat shock with ecoli. Is that correct? Will electroporation work? Or do I need to use cos sites and lambda phage...

22 January 2021 8,612 3 View

Bacteriophages very effective against infections. Some bacteriophages can be oncolytic and effective against cancers. How B_P recognize cancer cells?

Bacteriophages very effective against infections. Many of them such as anti-Staphylococcus or anti-Streptococcal widely used in practical medicine. Some bacteriophages can be oncolytic and...

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Should i add ATG in the beginning of my polypetide sequence when i want to insert it in a phagemid?

Hi I want to make a recombinant m13 phage displaying my peptide as a fusion peptide on its surface pVIII. Should i add ATG at the beginning when i want to insert it in phagmid vector? Previously,...

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Does anyone experience E coli lysis while seed culture?

Hi, I am currently working with BL21(DE3)PLySs and PRSET A vector. Out lab frequently transformation and culture E Coli for protein purification. But recently, even though there is colony after...

13 December 2020 9,167 9 View

Again, what are the bands of extracted plasmid?

I used Trans1-T1 phage resistant chemically competent cells to extracted lentiviral gRNA expression vectors. And there were bands near 2000bp and smear on 1% argrose gel. Did the smear come from...

25 November 2020 858 2 View

I alway get serval big plaques even with very high concentration of bacteriophages. how to get well-distributed plaques with double layer agar method?

I used MS2 (ATCC 15597-B1) strain to do my experiment. I always get serval big plaques (like the picture I attached), but not a plate which has a lot of small plaques I saw from other research....

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Suggest me a best filamentous m13 vector to generate scfv using phage display method?

i went through many literature to find out the exact viral vector but it was generally mentioned m13 vector in the papers not specific about the name, can anyone suggest me which m13 vector can...

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