I'd like to differentiate Macrophages, Fabroblasts, and Epithelium in a tumor sample through Flow Cytometry. Could anyone please give me some advice?
how come dsDNA from plasmid become ssDNA genome in AAV after packaging
11 December 2018 3,063 1 View
Biolegend Cell Surface Immunofluorescence Staining Protocol uses 15 ml of cell staing buffer to resuspend the cell. Is it necessary? Here is the link:...
10 November 2018 5,959 7 View
For RNA-seq, what level of gene expression in TPM would be considered as expressed and can be detected by IF or FACS? I've seen a situation in which the level of CD68 is about 50(TPM) detected by...
01 January 1970 2,069 3 View
I have a bulk RNA-seq data of a tissue, is it possible to tell if there exists macrophage or fibroblast by analyzing this data?
01 January 1970 3,117 5 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...
21 February 2021 8,548 7 View
Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?
21 February 2021 4,230 8 View
I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...
20 February 2021 2,504 1 View
18 February 2021 4,214 3 View