I have a bulk RNA-seq data of a tissue, is it possible to tell if there exists macrophage or fibroblast by analyzing this data?
use Cell Marker:
http://biocc.hrbmu.edu.cn/CellMarker/search.jsp
for macrophage it should be CD68
Hi Di Yang
you can also take a look at the PSEA algorithm (https://bioconductor.org/packages/release/bioc/html/PSEA.html) to extract data from RNA mix.
fred
Thank you all !
this is also great:
https://shenorrlab.github.io/bseqsc/vignettes/bseq-sc.html#data-preparation
The software xCell is used for enumerating cell subsets, including macrophages and fibroblasts, from bulk RNAseq data:
http://xcell.ucsf.edu/
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