Di Yang

5 Questions 5 Answers 0 Followers

Questions related from Di Yang

How does dsDNA in plasmid become ssDNA genome in AAV after packaging?

how come dsDNA from plasmid become ssDNA genome in AAV after packaging

12 December 2018 3,086 1 View

Is it necessary to add 15 ml cell staining buffer to resuspend the cell for flow cytometry?

Biolegend Cell Surface Immunofluorescence Staining Protocol uses 15 ml of cell staing buffer to resuspend the cell. Is it necessary? Here is the link:...

11 November 2018 5,973 7 View

What are the best markers to differentiate Marcrophages, Fabroblasts, and Epithelium?

I'd like to differentiate Macrophages, Fabroblasts, and Epithelium in a tumor sample through Flow Cytometry. Could anyone please give me some advice?

10 October 2018 7,798 0 View

Is it possible to identify cell type from a bulk RNA-seq instead of a single cell RNA-seq?

I have a bulk RNA-seq data of a tissue, is it possible to tell if there exists macrophage or fibroblast by analyzing this data?

01 January 1970 3,139 5 View

Why is RNA-seq positive but Flow cytometry negative?

For RNA-seq, what level of gene expression in TPM would be considered as expressed and can be detected by IF or FACS? I've seen a situation in which the level of CD68 is about 50(TPM) detected by...

01 January 1970 2,089 3 View