Hello,
I am trying to study insulin signaling by dual luciferase assays. I will have to transfect the cells with my reporter genes, for the same. What are the best mammalian cell lines, with good transfection efficiency, to study insulin signaling?
Note: I am not a big fan of HEPG2 cells.
Most pancreatic cancer cell lines should suffice for insulin signaling studies. Screening experiments can be conducted on a variety of cell lines to give more significant results, and you should probably use transfection reagents optimized for the cells as Christopher said. Here are some possible candidates and their transfection reagents:
- PANC-1 (https://altogen.com/product/panc-1-transfection-reagent-non-endocrine-pancreatic-cancer/)
- Mia-PaCa2 (https://altogen.com/product/mia-paca-2-transfection-reagent-pancreatic-carcinoma/)
- BxPC-3 (https://altogen.com/product/bxpc-3-transfection-reagent-pancreatic-cancer-cells/)
Each cell line has a large background of research, and probably some related to insulin signaling as well.
Hello Shruthi.
1.1B4 human cell line from Sigma (Product 10012801-1VL) or
Human pancreatic cells - SV40 from abm (Cat.No: T0157).
Regards.
Hi Shruthi,
You may use:
1.1B4-Human
BCT3-Mouse
depending upon your constructs.
There is a French group called Endocells that has generated a stable beta cell line (ENDOC BH1). I remember the MTA was very extensive and the cells were very expensive.
There is also a mouse line I always wanted to work with called Beta TC6 that has a transfection reagent that has been optimized for it. http://ezbiosystems.com/view2.asp?d_id=228
Luciferase was not easy to optimize in pancreatic cells in my hands. I'm happy to share more information about what I did if you'd like.
Most pancreatic cancer cell lines should suffice for insulin signaling studies. Screening experiments can be conducted on a variety of cell lines to give more significant results, and you should probably use transfection reagents optimized for the cells as Christopher said. Here are some possible candidates and their transfection reagents:
- PANC-1 (https://altogen.com/product/panc-1-transfection-reagent-non-endocrine-pancreatic-cancer/)
- Mia-PaCa2 (https://altogen.com/product/mia-paca-2-transfection-reagent-pancreatic-carcinoma/)
- BxPC-3 (https://altogen.com/product/bxpc-3-transfection-reagent-pancreatic-cancer-cells/)
Each cell line has a large background of research, and probably some related to insulin signaling as well.
Christopher Day , could you please tell me more of what you did? Thanks!
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