10 Questions 12 Answers 0 Followers
Questions related from Shruthi Gobbooru
Hi, I am trying to find any protocol, where people have studied/observed contact inhibition of proliferation, upon the addition of any ligand/antibody. Do you know of any such protocol/study? TIA.
08 August 2018 6,216 0 View
Hello all, does anyone know what's the doubling time (time for completion of one complete cell cycle) of Hep3B cell line ( ATCC® HB-8064™) ? (I've searched ATCC but didn't find any information)....
05 May 2018 1,040 3 View
Hello, I am trying to study insulin signaling by dual luciferase assays. I will have to transfect the cells with my reporter genes, for the same. What are the best mammalian cell lines, with good...
03 March 2018 4,111 7 View
Hello all, I am trying to study growth-regulating effects of Insulin in mammalian cells, by dual luciferase assays. I worked on HeLa cells but the results are not reproducible, even when I do the...
02 February 2018 8,900 3 View
Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...
11 November 2017 522 5 View
I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...
03 March 2015 1,827 3 View
Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...
03 March 2015 3,972 28 View
Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...
03 March 2015 6,556 4 View
I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...
12 December 2014 5,601 4 View
Hello everyone! I am testing the effect of certain compounds on cell proliferation by MTT assays. We usually analyse the data using Excel followed by Graphpad prism in our lab. I sometimes get...
09 September 2014 2,715 5 View
