Doubling time of Hep3B cells?

More Shruthi Gobbooru's questions See All

Ligand/antibody to study contact inhibition of proliferation?

Hi, I am trying to find any protocol, where people have studied/observed contact inhibition of proliferation, upon the addition of any ligand/antibody. Do you know of any such protocol/study? TIA.

07 August 2018 6,173 0 View

What are the best mammalian cell lines to study insulin signaling?

Hello, I am trying to study insulin signaling by dual luciferase assays. I will have to transfect the cells with my reporter genes, for the same. What are the best mammalian cell lines, with good...

02 March 2018 4,047 7 View

Do HeLa cells have insulin receptors?

Hello all, I am trying to study growth-regulating effects of Insulin in mammalian cells, by dual luciferase assays. I worked on HeLa cells but the results are not reproducible, even when I do the...

01 February 2018 8,857 3 View

Can you please suggest a good bioinformatics textbook/guide for gaining expertise on multiple sequence alignments?

Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...

10 November 2017 480 5 View

Can anyone explain to me about tagged western blotting?

I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...

02 March 2015 1,787 3 View

How can I induce DNA damage using Temozolomide?

Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...

02 March 2015 6,510 4 View

How can I minimize the air bubbles during a western blot wet transfer?

Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...

02 March 2015 3,893 28 View

What is the BRCA status of the colon cancer cell line HCT116?

I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...

11 December 2014 5,552 4 View

Can anyone help with plotting EC50 using Graphpad Prism: Incomplete sigmoidal curves?

Hello everyone! I am testing the effect of certain compounds on cell proliferation by MTT assays. We usually analyse the data using Excel followed by Graphpad prism in our lab. I sometimes get...

08 September 2014 2,618 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Kou Hayakawa

Amended on 3 June 2018

It seems to be difficult to assess doubling time, though bacterial doubling time seemes to be determined by turbidimetrically by using a nepherometer (please see file; Resistance to Hg).

In the case of HepG2, I have used volume measurement to estimate the growth rate (please see file; HepG2 fucoidan).

Increase of cell protein may be measured by using reliable HPLC-Surfactant-SEC method (please see file; HPLC-Surf-SEC protein determination method).

Further, doubling time may be changed by adding growth factors. Doubling time has surely decreased in Caco-2 cells by adding 5 μg/mL of biotin into the culture medium (please see file; JCB Fucoidan transport).

Biotin, lipoic acid (only to human cells), and oligo saccharide (such as nigero-oligosaccharide) are effectively decreased doubling times on HepG2, HuH-7, and HuH-6 (our unpublished observation at Gunma University, Maebashi, Gunma, Japan). Mechanism of oligo saccharide on growth rate is not clearly undestood at this time, but oligo saccharide may be a direct source for the glycochain biosynthesis of glycoproteins.

Growth promoting effect of oligo saccharide on bacteria of Bifidobacterium has been already well known. Please see the file "Oligosaccharide Growth".

Amos Bairoch

As you will see from the relevant Cellosaurus entry:

https://web.expasy.org/cellosaurus/CVCL_0326

The PDT is ~40-50 hours according to DSMZ

Rafael Franco

I wonder whether you are asking for doubling time when cells are already growing or just want to know when they will start growing when you thaw an aliquot of frozen cells. If the latter please tell for some specific advice when difficulties arise to "start" growth.