Hi,
I am trying to find any protocol, where people have studied/observed contact inhibition of proliferation, upon the addition of any ligand/antibody. Do you know of any such protocol/study? TIA.
Hello all, does anyone know what's the doubling time (time for completion of one complete cell cycle) of Hep3B cell line ( ATCC® HB-8064™) ? (I've searched ATCC but didn't find any information)....
04 May 2018 1,061 3 View
Hello, I am trying to study insulin signaling by dual luciferase assays. I will have to transfect the cells with my reporter genes, for the same. What are the best mammalian cell lines, with good...
02 March 2018 4,133 7 View
Hello all, I am trying to study growth-regulating effects of Insulin in mammalian cells, by dual luciferase assays. I worked on HeLa cells but the results are not reproducible, even when I do the...
01 February 2018 8,925 3 View
Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...
10 November 2017 543 5 View
I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...
02 March 2015 1,847 3 View
Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...
02 March 2015 6,579 4 View
Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...
02 March 2015 4,036 28 View
I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...
11 December 2014 5,626 4 View
Hello everyone! I am testing the effect of certain compounds on cell proliferation by MTT assays. We usually analyse the data using Excel followed by Graphpad prism in our lab. I sometimes get...
08 September 2014 2,745 5 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer
30 July 2024 3,354 1 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View
In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...
27 July 2024 201 0 View
Women also often find it easier to fantasise when self-pleasuring than in sex with a partner. The immediacy of someone else’s needs actually inhibits the expression and satisfaction of their own....
26 July 2024 8,351 2 View