Doubling time of Hep3B cells?

04 May 2018 944 3 View

What are the best mammalian cell lines to study insulin signaling?

Hello, I am trying to study insulin signaling by dual luciferase assays. I will have to transfect the cells with my reporter genes, for the same. What are the best mammalian cell lines, with good...

02 March 2018 4,047 7 View

Do HeLa cells have insulin receptors?

Hello all, I am trying to study growth-regulating effects of Insulin in mammalian cells, by dual luciferase assays. I worked on HeLa cells but the results are not reproducible, even when I do the...

01 February 2018 8,857 3 View

Can you please suggest a good bioinformatics textbook/guide for gaining expertise on multiple sequence alignments?

Hello all, I am looking for recommendations on a good bioinformatics textbook/guide which helps me gain sound background knowledge on multiple sequence alignments. A part of my research is to...

10 November 2017 480 5 View

Can anyone explain to me about tagged western blotting?

I am curious about the tagged western blotting, where, proteins are probed on a nitrocellulose/PVDF membrane with HIS-tag/GST-tag primary antibody initially, and later with a corresponding...

02 March 2015 1,787 3 View

How can I induce DNA damage using Temozolomide?

Hi all, I am currently studying activation of PARP after inducing DNA damage. The method I follow is: 1) exposing tumor cells (HCT116, HeLa cells) and MEFs (WT and S12) to DNA damaging agent...

02 March 2015 6,510 4 View

How can I minimize the air bubbles during a western blot wet transfer?

Hello everyone, I am right now facing the issue of air bubbles during the western blot wet transfer method. It is so frustrating that some silly air bubbles mess up the whole results. This is what...

02 March 2015 3,893 28 View

What is the BRCA status of the colon cancer cell line HCT116?

I am trying to put together the BRCA and p53 status of various cell lines like HeLa, WT/S12 MEFs, HCC1937, MDAM231, MCF7, HCT116. I was able to get the statuses of every other cell line, but just...

11 December 2014 5,552 4 View

Can anyone help with plotting EC50 using Graphpad Prism: Incomplete sigmoidal curves?

Hello everyone! I am testing the effect of certain compounds on cell proliferation by MTT assays. We usually analyse the data using Excel followed by Graphpad prism in our lab. I sometimes get...

08 September 2014 2,618 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View