Dear All
I used wavelength of 220 to measure carbofuran, but sometimes there are some other peaks although it does not contain any impurities. I am afraid of interference of these peaks with its intermediates when doing degradation. What is the reason for that and what are the best conditions for measuring carbofuran and its intermediates using HPLC?
Regards,
Dear Amal,
It is very risky to analyse carbofuran at 220 nm UV. In samples (complex mixtures) you will always have other coeluting peaks which could give a false positive signal. I highly recommend you to use a MS detector such as a triple quad or TOF to be more selective.UV is also less sensitive then MS detectors.
If you have only an UV detector at your disposal I would try to use a few extra wavelengths and calculate the signal ratio to avoid fals positive signals.
But still the best option is a MS detector.
Kind regards,
Gerrit
Dear Dr. Gerrit
Thanks for reply, but I will measure analytical sample of carbofuran not complexes.
Regards,
We currently detect carbofuran between 275 and 280 nm in biological samples (plasma / urine) with no interferences. MS is definitely more sensitive (even GCMS works for carbofuran)
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