How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Cross-lagged panel analysis and sample size?

I want to estimate the causal relationship between two latent variables over time using structural equation modelling. both latent variables are measured according to different indicators...

31 January 2021 3,777 5 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

SEM for panel data ?

I'm trying to find the effect of smart cities on sustainable development. I collected data for several indicators of smartness of a city for 15 cities over 12 years. seems like panel data (GMM...

12 January 2021 9,914 5 View

How to prepare H2O2 solution or certainly how can we prepare 30% w/v H2O2 solution?which solvent?

Inorder to enhance the photocatalytic activity of magnetic nano photocatalysts like zinc ferrite we can use H2O2.

10 November 2019 5,780 3 View

Does anyone know where i can purchase drug-permeable e. coli?

I need an e. coli strain that's membrane is permeable to antibiotics.

10 October 2019 2,177 3 View

No title

18 May 2019 5,751 2 View

Can anyone suggest me to set up a suitable basis set for theoretical calculations of organometallic complex?

I have recently installed Gaussian 16 for dft calculations. I have no good idea about suitable basis set? How to specify basis set for heavy atom and light atoms? YouTube tutorial mainly related...

16 February 2019 9,237 4 View

What are the tips to be considered for peptide encapsulation in liposome?

I am working on nanoliposome formulation to encapsulate a synthetic peptide , I have tried many ways for encapsulation, but after dialysis there wasn't any peptide in liposomes which I analyzed by...

02 December 2018 8,010 7 View

How do I analyse my luciferase data?

Hi I'm looking for some help in analysing my luciferase data. I have a transcription factor and then a range of proteins I think will influence transcription factor activity. For each...

08 November 2018 4,617 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How to run repeated measures in mvabund?

I have a dataset of fish species abundances collected from 6 different habitats over the course of 30 years. Fish were collected multiple times per year and environmental variables were recorded...

21 February 2021 3,838 1 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to get rough estimation of the concentrations of Oxygen and moisture inside the glove box?

Dear colleagues, I was just wondering if you can suggest a good method to get a rough estimation to the concentration of O2 and H2O inside the glove box as unfortunately, our glove box doesn't...

17 February 2021 1,012 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View