I am trying to identify by flow cytometry osteogenic and chondrogenic lineages differentiated from MSCs in human BM. What would be the best markers (intracellular or extracellular) for this assay?
I thought about ALP for osteogenic and SOX9 for chondrogenic but I don’t know whether those are the right targets.
Thank you!
http://www.sciencedirect.com/science/article/pii/S1873506113001359 this might help you
ALP and Sox9 are both quite early markers for their respective lineages, but are fairly specific. I don't know how well you would get on with flow for Sox9 though since its a nuclear protein, but the later markers that are usually examined by PCR are all matrix proteins which are not very practical to assay either. It's also challenging to get single cell suspensions from cells that have differentiated well!
I did PCR and microarray for chondrogenesis using fat pad derived MSCs.
Genes such as SOX9, GAGs and Col2 are more specific.
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