How can I Make a final concentration of 1ug/ml, 2ug/ml, 4ug/ml,...32ug/ml of vancomycin? Does volume of agar affects the concentration?

More Felix Adi's questions See All

Please what is the best solvent to dissolve heme?

I have tried NaOH and NH3 so far but can't dissolve well.Thanks for the assistance. I can be contact personally on this email:[email protected]

04 January 2021 5,528 2 View

PVDF membrane degradation?

Hi, I'm performing western blots on different samples and I'm facing a some problems after eletrotrasnfer with PVDF membranes. In general, during the incubations with the primary and secondary...

18 November 2020 6,412 7 View

how can i have the optical Mode size in COMSOL?

hello, Im using the Electromagnetic Waves Beam Envelops interface in order to simulate two seperate structures: one is a waveguide inside silicon, second is just propagation inside a bulk of...

10 October 2020 6,184 2 View

What would happen if the work function (WF) of the cathode is lower than the acceptor LUMO?

Dear colleagues, Now I am working on BHJ solar cells with inverted structure. I have a case where the cathode's WF (~3.8 eV) is lower than acceptor LUMO (~3.9 eV). What would happen if the...

18 August 2020 9,367 3 View

How to implement lower partial moment of order 1 for given stocks in excel sheet?

I am working on portfolio optimization using lower partial moment of order 1, can someone help me how to implement LPM-1 in excel sheet using "tau" as my threshold value as 0.00% and order (n) as...

05 July 2020 2,510 1 View

Do female sandbar sharks school?

I am looking for literature regarding sexual segregation in sandbar sharks (carcharhinus plumbeus). I was told once that males school but females are solitary, and I cannot find a reference...

07 May 2020 2,537 2 View

How can I adjust the tilt angle and the distance between the absorbed small molecules and the substrate?

I am here using the avogadro program for the construction of molecular structures, before doing DFT calculations. I use H2O and Graphene as small molecules and their substrates.

07 May 2020 4,661 3 View

What to do when you found a published paper that doesn't belong to you but has your name as the author?

I found a published paper online (attached here) that bears mine and colleague's names as authors but the paper doesn't belong to us. The work was claimed to be carried out in Kenya. We are in...

22 March 2020 4,860 7 View

Do you know a (validated) instrument for the assessment of state embarassment?

I've found quite a few scales for vicarious and personal embarassibility as a personality trait so far (i. e. Kelly & Jones, 1997; Maltby & Day, 2000; Uysal et al., 2014...). As I'd like...

24 February 2020 8,142 4 View

Are Peak Areas of different silylations comparable?

If I have an analyte, that has different silylations, like 2-TMS and 3-TMS, how can I compare the peak areas? Can I simply sum them up? What if theres a different ratio of 2-TMS/3-TMS in my...

03 December 2019 273 4 View

Due to the increase of antibiotic resistance, Aggregatibacter actinomycetemcomitans is resistant to what antibiotics?

Since resistance to ampicillin, vancomycin, and etc.. is common for A. actinomycetemcomitans, is there any recent updates that A. actinomycetemcomitans can be resistant to a different antibiotic...

30 September 2020 341 3 View

Why Lactobacillus acidophillus is sensitive to Vancomycin but L. rhamnosus and casei are intrinsically resistant?

Does it have, anything to do with their fermentation status? I know that in several species of LAB, the terminal d-alanine residue is replaced by d-lactate or d-serine in the muramylpentapeptide,...

19 September 2020 3,242 2 View

What are the possible ways to differentiate between VRE and Pediococcus?

I am using a media that is selective for vancomycin-resistant enterococci, but it keeps giving me Pediococcus (since Pediococcus is intrinsically resistant to vancomycin and has similar...

26 September 2019 403 4 View

Would you please suggest a suitable and uptodate reference to check the volume of distribution of each drug?

I want to have the volume of distribution of vancomycin but I found variable amounts between 0.5, 0.7, and 0.9 L/Kg in different references. What is the best value in critically ill patients? And...

15 August 2019 5,955 3 View

Would you please suggest a suitable and uptodate reference to check the volume of distribution of each drug?

I want to have the volume of distribution of vancomycin but I found variable amounts between 0.5, 0.7, and 0.9 L/Kg in different references. What is the best value in critically ill patients? And...

07 August 2019 6,763 3 View

How to measure the concentration of vancomycin coated on Ti surface?

Hi everyone, I am working on covalently coating vancomycin on the surface of titanium rod, but have a question about how to measure the concentration of vancomycin in the coating. Many references...

04 May 2019 1,779 1 View

Can i use HFIP (1,1,1,3,3,3-Hexafluoroisopropanol ) as solvent system?

for dissolving the Polycaprolactone, collagen, and Vancomycin hydrochloride drug? please suggest?

31 December 2018 1,832 4 View

How can i dissolve the Vancomycin hydrochloride drug in PCL-collagen solution?

solvents used for PCL-collagen is chlorofrom and acetic acid please suggest?

31 December 2018 770 0 View

How entrapped Vancomycin can be released from a supramolecular gelatin nanoparticles? i want to measure the total entrapped content of Vancomycin?

Gelatin to Vancomycin ratio 10:2 respectively; in PBS

05 June 2018 8,492 0 View

When should I change the water including antibiotics cocktail for antibiotics-induced microbiota depleted mice?

When should I change the water including antibiotics cocktail for antibiotics-induced microbiota depleted mice (AIMD mice)? Hello, fella researchers. I am trying to make AIMD mice for my...

13 May 2018 6,784 6 View

Michael J. Benedik

You just need to make a concentrated stock solution and add different amounts of the stock to your medium.

It is not clear to me what you are asking about the agar, but you have given final concentrations of the antibiotic so it should not matter how much media is going into each plate if not precisely uniform.

Satwik Majumder

Hi Felix Adi , serial dilution is the best way to check resistance. Use liquid media (such as MHB).

These papers may help you with the method of serial dilution:

You may use the same for antibiotics.

Article Prevalence and mechanisms of antibiotic resistance in Escher...

https://www.sciencedirect.com/science/article/abs/pii/S1011134422000641