My lab has some silver acetate or gold chloride staining on our scale and we want to remove it. Any help would be appreciated!
I've been having reproducibility issues with MHII for antibiotic resistance assays so I found a paper that showed ISB is comparable to MHII and the distributor says ISB is touted for its...
01 January 1970 1,425 1 View
I'm having times where I get a 2.8 OD600 reading on a spectrophotometer and other times I get 0.8. I read on previous literature my enzyme is negatively regulated by quorum sensing, but this is...
01 January 1970 6,447 1 View
The instructions say to autoclave to 121C for 10 minutes and not to overheat. Does that mean if you autoclave for 20 or 30 minutes, it would compromise the integrity of the media, specifically the...
01 January 1970 4,454 0 View
Is there any difference if you do it with L broth vs MHII broth? I know MHII has starch, which neutralizes toxins and has better diffusion on agar, but what if you use L broth instead?
01 January 1970 1,932 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I imagine the battery drives the graphite into a carbocation, then chloride is oxidized to donate the electron to the carbocation, but what pushes the lost electrons of chloride to be shared...
03 August 2024 5,384 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Please note it
29 July 2024 4,920 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Here, I have used Ag/AgCl as reference electrode and 0.5M KOH.
29 July 2024 4,538 3 View