The instructions say to autoclave to 121C for 10 minutes and not to overheat. Does that mean if you autoclave for 20 or 30 minutes, it would compromise the integrity of the media, specifically the cations?
My lab has some silver acetate or gold chloride staining on our scale and we want to remove it. Any help would be appreciated!
24 October 2023 7,936 0 View
I've been having reproducibility issues with MHII for antibiotic resistance assays so I found a paper that showed ISB is comparable to MHII and the distributor says ISB is touted for its...
01 January 1970 1,425 1 View
I'm having times where I get a 2.8 OD600 reading on a spectrophotometer and other times I get 0.8. I read on previous literature my enzyme is negatively regulated by quorum sensing, but this is...
01 January 1970 6,447 1 View
Is there any difference if you do it with L broth vs MHII broth? I know MHII has starch, which neutralizes toxins and has better diffusion on agar, but what if you use L broth instead?
01 January 1970 1,932 3 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container
30 July 2024 7,326 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
Thanks
22 July 2024 1,143 3 View
I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?
12 July 2024 9,904 1 View
DNA isolation requires many freshly made buffers. So is it necessary to autoclave each vessel used, before preparing buffers in them?
10 July 2024 7,526 3 View
Les géopolymères offrent plusieurs propriétés intéressantes qui peuvent être exploitées dans diverses applications innovantes : 1. Construction durable : - Bétons à faible empreinte carbone -...
10 July 2024 7,457 1 View
If we want to introduce a material similar to polymethyl methacrylate "with the characteristics mentioned in the next paragraph" that can be added to a precursor and create a composite, which...
03 July 2024 2,371 1 View
We are fabricating a tandem structure where a ~50 nm thick WO3 film is deposited between two metal films using DC sputtering. We use a mixture of Ar and O2 gases at different flow rates while...
01 July 2024 2,686 2 View
I want to perform DFT calculations for a triple cation mixed crystal. Which software is preferable and free?
28 June 2024 5,255 2 View