David Danh Ngo

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Questions related from David Danh Ngo

What are some ways to remove silver acetate or gold chloride staining?

My lab has some silver acetate or gold chloride staining on our scale and we want to remove it. Any help would be appreciated!

25 October 2023 7,910 0 View

Antibiotic resistance using L Broth vs MHII Broth

Is there any difference if you do it with L broth vs MHII broth? I know MHII has starch, which neutralizes toxins and has better diffusion on agar, but what if you use L broth instead?

01 January 1970 1,917 3 View

Cation-Adjusted Mueller Hintion Autoclaving

The instructions say to autoclave to 121C for 10 minutes and not to overheat. Does that mean if you autoclave for 20 or 30 minutes, it would compromise the integrity of the media, specifically the...

01 January 1970 4,431 0 View

Does Iso-Sensitest Broth yield more reproducible results than Mueller Hinton II (cation adjusted)?

I've been having reproducibility issues with MHII for antibiotic resistance assays so I found a paper that showed ISB is comparable to MHII and the distributor says ISB is touted for its...

01 January 1970 1,412 1 View

If an antibiotic resistance gene is regulated by quorum sensing, would that cause inconsistent results?

I'm having times where I get a 2.8 OD600 reading on a spectrophotometer and other times I get 0.8. I read on previous literature my enzyme is negatively regulated by quorum sensing, but this is...

01 January 1970 6,426 1 View