What are some suitable buffers and at what pH can Rhizobium cells be stored to increase survival for a long time (e.g 1 month)?

More Toby Adjuik's questions See All

How to clear up DCX staining for IHC?

I am working from a robust protocol I use routinely and have a good workflow on the microscope so am certain the issue is the primary antibody. I have tried three different DCX antibodies (two...

14 February 2024 9,377 0 View

I'm using quantum espresso Projwcf.x to find PDOS, how do i determine which sublevel each column is?

I'm using quantum espresso Projwcf.x to find PDOS, i need to find orbital sublevel contributions from the output file but I can't seem to work out which column gives which sub level. For example...

18 June 2023 3,766 2 View

QPCR NTC's positive with variation in duplicates?

Hi all, I am getting positive NTC's on my qPCR plates. I have determined it is likely due to primer dimer and not contamination.This is all good but I am having trouble understanding a few...

25 October 2022 1,369 0 View

How can REAP or other EIS data be used to determine the actual lifespan of zinc-rich paint?

Is there a model or other technique to estimate the useful lifespan of zinc-rich paint based on EIS data? I will not be able to undertake long-term testing of actual corrosion rates for...

28 April 2020 7,972 10 View

Triangulation of gibbons using ArcGIS?

I am trying to analyse gibbon auditory point transect data to estimate gibbon density. The traditional method of triangulation analysis is to map out the data by hand to determine how many...

27 April 2020 8,159 4 View

Does any have experience of using the Pyromark Q48?

We are measuring methylation at the POMC gene on human DNA. We are getting good results with out PCR but are consistently having issues with low peak height and baseline drift on the sequencing...

01 October 2019 4,527 9 View

Lucy 2.0 Question - How do I cut low-quality ends off Sanger reads?

I have tried playing with the settings, changing values for Error (Average, ends) and bracket window - as the software suggests - and I can't get the settings right to remove strings of bases (say...

06 May 2018 8,383 0 View

RNA extraction with 96 well plate kits?

Hi, Has anyone used Qiagen's RNeasy 96 Universal tissue kits? How did you find it? Also, what about Macherey Nagel's NucleoSpin™ 96 RNA? Which is better? I have 6000+ bees to homogenate and...

30 June 2016 5,306 3 View

Dry shipper recommendation?

Can anyone recommend a dry shipper in the UK. I'm looking to store ~400 samples, mostly in 1.5ml tubes, though some will be bigger. Not bothered about correct storage on straws etc, just going to...

31 January 2016 2,992 1 View

What computer program should I use to compare plant communities?

I'm doing an inventory of vascular plants in a piedmont gorge (USA) and I'm going to do some transects for community comparisons and analysis. I'll compare communities on different facing slopes...

20 November 2015 7,913 10 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to assess the osmotic power of a substance?

Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...

06 August 2024 977 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View