What are some simple methods for conventional m7g cap detection?

27 July 2021 0 4K Report

I want to analyze efficiency of capping enzyme, expressed and purified by our group. What simple and reliable assays would you suggest? Thank you in advance!

Badges
Science topic

More Daniil Shmidt's questions See All

How do I check the performance of my PEI?

The expiration date of my PEI transfection reagent is out and I do not know how to check it. I only use siRNA in my work and I can't figure out what I'm doing wrong or what's wrong with PEi. Can I...

04 December 2023 4,783 2 View

Is it possible to test siRNA transfection by PCR?

I am performing siRNA transfection to inhibit a certain matrix RNA. Does it make sense in this case to determine the reduction of this RNA by reverse transcription on PCR?

30 October 2023 1,494 4 View

What does "topeyed" mean for southern topeyed lampreys?

I have a certain misunderstanding with this term, for a literal translation into Ukrainian (English speakers, please, because I don't really understand the context). I look at the photo and the...

05 July 2023 5,056 0 View

What is the optimal particle size for soil biomining?

Is there an optimal particle size that is suitable for the extraction of metals (iron, titanium, aluminum, REE) from soil? If the particle size is small (

26 June 2023 8,296 0 View

Most popular information economics theories?

Dear colleagues, Whats your opinion on the top-10 most popular/basic theories in the field of information (technology) and economics? That answer the question "how information collection,...

22 April 2023 8,538 6 View

Does the absorption coeficient of laser radiation by a material depend on beam polarization?

If it does, how does the situation look for crystalline and amorphous materials? Suggestions of papers/books to educate me on this topic are appreciated.

08 February 2023 2,822 1 View

Where can i buy a black diffuse reflectance paint?

I need to make low cost reflectance calibration samples. I plan to apply special paints on a plywood board. For high reflectance, BaSO4 paint works well, but I can't find low (10%) reflectance...

07 November 2022 5,059 3 View

Indexes of biological weathering?

Are there indexes for calculating the biological weathering? Or do we have other ways of determining the intensity of biological weathering? It would be appropriate if they were based on the...

26 July 2022 2,775 0 View

Could you please recommend a source that explains a basic scheme of quantum key distribution in a backbone system?

A scheme that includes different elements of QKD and their interrelations, like servers, random number generator, photon counters, etc.

14 October 2021 1,533 2 View

How can we calculate the habitable zone for a star?

In textbooks on astronomy, we can find information that the radius of the habitable zone is influenced by the luminosity of the star, the sperctral class, and metallicity. But are there any...

17 June 2021 7,279 4 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View

I need a reliable source or an example supported by excel sheet to understand Fuzzy Vikor?

27 July 2024 5,916 1 View

Is a reliability test necessary in my survey on translations?

Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...

24 July 2024 8,245 5 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can you suggest reliable procedures to get displacements from accelerations in frequency domain ?

I have identified many solutions. I need suggestion from somebody with application experience of this topic to identify the most reliable and robust procedure.

21 July 2024 3,465 5 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View