What is the maximum overall column length suitable for LC-MS using a 50 mm HILIC column?

Curious about your research experiences. Ideally this is for ESI-QTOF electrospray ionisation. This will differ based on type of columns and equilibration as per dimensions, flow rate and size of...

09 October 2017 5,453 6 View

Are there any literature sources on coupling of HILIC column to another separate stationary phase?

08 September 2017 8,659 6 View

What research techniques have you learned over the years allowing you to quickly finish research?

For me cite them right website for referencing got lab reports done easy with references. Learned web of science makes results so narrow and easy to find. Google scholar let me find cited articles...

08 September 2017 5,953 2 View

I want to test my HILIC column with different mobile phase %. Do i need to equilibrate 2 hrs per mp %?

the water layer would have formed using 85%. Is the same amount of water layer formed by each acetonitrile %.what I am asking is for 97% if I equilibrat for 2 hrs then can I equilibrate for 30 to...

08 September 2017 7,296 11 View

How much of a water layer is required in HILIC column to effectively bind hydrophobic compounds?

,Tried 3% water layer but equilibration time is time consuming. Analyte had a curved out looking shape such as an upside boat that continued for 2minutes to reach the baseline. steep then smooth...

08 September 2017 4,633 9 View

For my HPLC UV detection can I apply 0.1% formic acid and 10mM Ammonium acetate at pH 4 to see what effect the buffer combo can have in HILIC?

I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the...

08 September 2017 617 4 View

What are some good examples of articles for moderately polar compounds separated by HILIC?

LogP

08 September 2017 720 5 View

I have an impurity from a purchased bacterial standard. What methods do you use to analyse if it is a standard besides HPLC-UV detection?

08 September 2017 7,121 2 View

What are the typical To void volume markers used for mixed mode chromatographic columns that do not retain hydrophobically/ hydrophilically?

This situation can be applied to 2D chromatography. If there is a T-piece can two To void volume markers be used to measure that volume for sample to reach the detector. Some research applies...

08 September 2017 9,947 5 View

Are there any infra red detectors for HPLC or UV+IR detector for liquid or gas or supercritical fluid chromatography?

Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...

08 September 2017 7,457 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

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01 March 2021 3,905 5 View