Preferably free.
Curious about your research experiences. Ideally this is for ESI-QTOF electrospray ionisation. This will differ based on type of columns and equilibration as per dimensions, flow rate and size of...
09 October 2017 5,600 6 View
the water layer would have formed using 85%. Is the same amount of water layer formed by each acetonitrile %.what I am asking is for 97% if I equilibrat for 2 hrs then can I equilibrate for 30 to...
08 September 2017 7,441 11 View
,Tried 3% water layer but equilibration time is time consuming. Analyte had a curved out looking shape such as an upside boat that continued for 2minutes to reach the baseline. steep then smooth...
08 September 2017 4,792 9 View
Anal Chim Acta. 2017 May 15;967:12-32. doi: 10.1016/j.aca.2017.01.060. Epub 2017 Feb 7. Recent advances in stationary phases and understanding of retention in hydrophilic interaction...
08 September 2017 8,806 6 View
For me cite them right website for referencing got lab reports done easy with references. Learned web of science makes results so narrow and easy to find. Google scholar let me find cited articles...
08 September 2017 6,102 2 View
I read in Mass spec literature that buffer combinations are attempted. Are they only for gradient elution. Does this need mass spec to ionise the buffered solvent effectively. Will this cause the...
08 September 2017 834 4 View
LogP
08 September 2017 869 5 View
This situation can be applied to 2D chromatography. If there is a T-piece can two To void volume markers be used to measure that volume for sample to reach the detector. Some research applies...
08 September 2017 10,129 5 View
Which methods are the fastest and easiest to do to save time. The LC-MS is broken for now and awaiting repair otherwise I would fragment the impurity with different m/z ionisation. In the...
08 September 2017 7,261 2 View
Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...
08 September 2017 7,592 5 View
I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...
12 August 2024 862 0 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
