https://www.proteinatlas.org/ENSG00000185499-MUC1/cell

https://www.creative-bioarray.com/

Hiba Riyadh Al-abodi That link only shows RNA expression.

https://www.proteinatlas.org/ENSG00000185499-MUC1/tissue

According to protein expression of MUC1 in various tissues from this link, we can see that any cell line origin from skeletal/heart muscle and hepatocyte should be MUC1 negative. Best luck

Amended on 14 December 2018

I have searched Mucin-1/CD227 in my human protein database in vain (please see file; HepG2 fucoidan). This may be due to famous Species Differences. The sepcies difference between human and mouse seems to be large. I have previously surprized that the species difference in biotinidase is also unexpectedly large between rat and mouse (please see file; J Chrom B Rat BIN LIP Km).

Humans express following CDs; i.e.,

CD1d, CD5 antigen-like/IgM-associated peptide, CD8-alpha, CD8-beta, CD13, CD14, CD20 antigen-like 7/Membrane-spanning 4-domains subfamily A member 10, CD21, CD29, CD30, CD40, CD44, CD45, CD49b, CD49d, CD51, CD55/Complement decay-accelerating factor, CD56, CD58, CD61, CD88, CD98, CD99, CD103/Integrin alpha-E/HML-1 antigen, CD104, CD107a, CD116/Granulocyte-macrophage colony-stimulating factor receptor alpha chain/GM-CSF-R, CD117, CD127, CD129, CD152, CD154, CD163, CD233/Anion exchange protein 1/Band 3 anion transport proteinSLC4A1, CD243, CD331, CD332.

By the way, mucins are detected in some human specimens; i.e.,

Human breast milk has Mucin-5AC/Gastric mucin/Mucin-5 subtype AC, tracheobronchial at 2.7 μg/mg of milk protein.

Healed hepatocyte HepG2 (cultured with fucoidan at 0.106 mg/mL for 3 days) has Mucin-16/Ovarian cancer related tumor marker CA125/Ovarian carcinoma antigen CA125 at 67.4 μg/mg of cell protein (6.74%).

Serum of GSD-1b patient (3y, female; alopecia has been healed by biotin therapy, after 2mo of the liver transplantation) has Mucin-6/Gastric mucin-6 at 10.8 μg/mg of serum protein (please see file; JMBT Alopecia).

Fetal hepatocyte Hc has expressed no mucin gene. The expressions of mucins in Human breast, Healed hepatocyte HepG2 and Serum of GSD-1b patient seem not to be linked to virus, and these expressions seem to be up regulated by the own expressing power of healthy mammary gland, normal healed-cultured HepG2 cells, and regenerating liver organ, respectively.

HCC tissue (named as No.6; who has happily survived) has Mucin 2/Intestinal mucin-2 at 10.6 μg/mg of tissue protein.

This tissue has Nucleocapsid protein (Measles virus/MeV) at 0.033, and RNA-directed RNA polymerase L/Protein L (Measles virus/MeV) at 18.4 μg/mg of tissue protein, respectively. There is no MeV in his LC tissue, and LC tissue has no Mucin.

Serum of healthy man (33y, male) has Mucin-5B/Cervical mucin/Sublingual gland mucin/Mucin-5 subtype B, tracheobronchial at 29.9 μg/mg of serum protein.

This serum has RNA-directed RNA polymerase L/Protein L (Measles virus/MeV) at 20.4 μg/mg of serum protein.

Interestingly, only HCC tissue (named as No.6) and Serum of healthy man (33y, male) uniquely have MeV.

Terefore, Mucins of HCC-liver tissue (named as No.6) and Serum of healthy man (33y, male) seem to be up regulated by Measles virus/MeV.

Kou Hayakawa Thank you very much! That was very helpful. Really appreciate your help.

The androgen-dependent, AR-positive LNCaP cell line is an epithelial cell line derived from a human prostate carcinoma.