What would be the difference if I use the Web server instead of dedicated server for protein modeling and docking?

Venkata Vamsi Bharadwaj Yallapragada @Venkata_Vamsi_Bharadwaj_Yallapragada2

04 April 2017 0 7K Report

We know that software tools like I-tasser etc have a web server and a standalone option too. Is there any difference to protein C-scores or efficiency or accuracy if you do it on standalone instead of web server?

Badges
Science topic

More Venkata Vamsi Bharadwaj Yallapragada's questions See All

Which (purified) protein's fluorescence would last longer? CFP or GFP or RFP?

I was wondering if anyone of these would have fluorescence that will last for weeks? (Purified protein)

01 February 2019 3,015 1 View

What are some MUC1 negative cell lines?

I'm looking cell lines that don't express MUC1. Any suggestions will be highly appreciated. Thank you

11 December 2018 3,822 7 View

Are there any small scale protein purification columns for His tagged proteins?

My sample would be around 100-500ul and I wanna purify His tagged proteins from it.

10 November 2018 746 4 View

When a protein is expressed in Ecoli, with Gaussia luciferase's secretion signal, is the secretion signal cleaved before the protein gets secreted?

I am planning to express a recombinant protein in Ecoli and want it secreted extracellular (into the media). I have been reading that Gaussia's sec signal would work in prokaryotes as well. I...

10 November 2018 2,680 4 View

Is there any commercial kit to extract transfected plasmid DNA from mammalian cells?

I also tried searching for total DNA extraction from mammalian cells kit, couldn't find any. All the search results were for genomic DNA or ribosomal/mitochondrial DNA extraction. Do you know a...

09 October 2017 8,399 18 View

Has anyone used mini-circle plasmid system? Did you experience and practical difficulties while using them?

I am looking for antibiotic resistance free plasmid systems and came across this concept of mini-circles. I am wondering what would be the experience of someone who used them, or any practical...

03 April 2017 8,177 2 View

Can anyone suggest me the best computational tool for predicting antigen antibody affinity?

I have modeled the 3D structures of the antibody and the antigen, now I have several antibodies against the same antigen, I am looking for tools that would tell me which antibody has the maximum...

02 March 2017 1,618 3 View

Which is more reliable, I-Tasser or Rosetta?

I am currently trying to model my proteins (they are antibody fragments) I am looking for this comparison between the two major tools for protein model prediction. I either find people saying to...

02 March 2017 8,615 9 View

Gaussia luciferase at N terminus or C terminus of the protein? Which is better?

what are advantages/disadvantages of adding gaussia luciferase on N terminus than on C terminus of the protein of interest. and would it work if I attach the gluc signal peptide at the N- terminus...

02 March 2017 3,971 1 View

Can I use ethanol and glycogen to concentrate my RNA sample??

I have 10^5 cell mammalian culture and I have isolated total RNA from them, as a part of an important experiment, however the yield was very low. Though I have more than required amount, the...

03 April 2016 2,509 6 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Which Scopus Journal provides the most affordable fees?

"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....

10 August 2024 8,621 1 View

Seeking Advice on Viability and Execution of Undergraduate Thesis Topic?

Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...

10 August 2024 8,992 0 View

Is it possible to use the Fused Deposition Modeling (FDM) to additively manufacture interconnected porous structure generation of >100-200 micrometer?

Usually, additive manufacturing techniques like SEBM, SLS, and SLM are used for interconnected porous lattice structure generation with sizes of >100–200 micrometers. Can the Fused Deposition...

09 August 2024 7,892 0 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Who will be moral responsible for the death of thousands of people in the event of an earthquake?

Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...

08 August 2024 6,134 12 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Can you suggest reliable sources defining "3D mesh" and "3D city models"?

Dear fellow researchers, I am currently working on a paper where I need to provide a reliable reference that defines and distinguishes between 3D mesh models and 3D city models. Although I am...

06 August 2024 9,986 2 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View