Which (purified) protein's fluorescence would last longer? CFP or GFP or RFP?

I was wondering if anyone of these would have fluorescence that will last for weeks? (Purified protein)

01 February 2019 2,909 1 View

What are some MUC1 negative cell lines?

I'm looking cell lines that don't express MUC1. Any suggestions will be highly appreciated. Thank you

11 December 2018 3,718 7 View

Are there any small scale protein purification columns for His tagged proteins?

My sample would be around 100-500ul and I wanna purify His tagged proteins from it.

10 November 2018 639 4 View

When a protein is expressed in Ecoli, with Gaussia luciferase's secretion signal, is the secretion signal cleaved before the protein gets secreted?

I am planning to express a recombinant protein in Ecoli and want it secreted extracellular (into the media). I have been reading that Gaussia's sec signal would work in prokaryotes as well. I...

10 November 2018 2,542 4 View

Is there any commercial kit to extract transfected plasmid DNA from mammalian cells?

I also tried searching for total DNA extraction from mammalian cells kit, couldn't find any. All the search results were for genomic DNA or ribosomal/mitochondrial DNA extraction. Do you know a...

09 October 2017 8,308 18 View

Has anyone used mini-circle plasmid system? Did you experience and practical difficulties while using them?

I am looking for antibiotic resistance free plasmid systems and came across this concept of mini-circles. I am wondering what would be the experience of someone who used them, or any practical...

03 April 2017 8,084 2 View

Can anyone suggest me the best computational tool for predicting antigen antibody affinity?

I have modeled the 3D structures of the antibody and the antigen, now I have several antibodies against the same antigen, I am looking for tools that would tell me which antibody has the maximum...

02 March 2017 1,529 3 View

Which is more reliable, I-Tasser or Rosetta?

I am currently trying to model my proteins (they are antibody fragments) I am looking for this comparison between the two major tools for protein model prediction. I either find people saying to...

02 March 2017 8,480 9 View

Gaussia luciferase at N terminus or C terminus of the protein? Which is better?

what are advantages/disadvantages of adding gaussia luciferase on N terminus than on C terminus of the protein of interest. and would it work if I attach the gluc signal peptide at the N- terminus...

02 March 2017 3,892 1 View

Can I use ethanol and glycogen to concentrate my RNA sample??

I have 10^5 cell mammalian culture and I have isolated total RNA from them, as a part of an important experiment, however the yield was very low. Though I have more than required amount, the...

03 April 2016 2,361 6 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View