02 February 2019 1 3K Report

I was wondering if anyone of these would have fluorescence that will last for weeks? (Purified protein)

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What are some MUC1 negative cell lines?

I'm looking cell lines that don't express MUC1. Any suggestions will be highly appreciated. Thank you

11 December 2018 3,822 7 View

Are there any small scale protein purification columns for His tagged proteins?

My sample would be around 100-500ul and I wanna purify His tagged proteins from it.

10 November 2018 746 4 View

When a protein is expressed in Ecoli, with Gaussia luciferase's secretion signal, is the secretion signal cleaved before the protein gets secreted?

I am planning to express a recombinant protein in Ecoli and want it secreted extracellular (into the media). I have been reading that Gaussia's sec signal would work in prokaryotes as well. I...

10 November 2018 2,680 4 View

Is there any commercial kit to extract transfected plasmid DNA from mammalian cells?

I also tried searching for total DNA extraction from mammalian cells kit, couldn't find any. All the search results were for genomic DNA or ribosomal/mitochondrial DNA extraction. Do you know a...

09 October 2017 8,399 18 View

Has anyone used mini-circle plasmid system? Did you experience and practical difficulties while using them?

I am looking for antibiotic resistance free plasmid systems and came across this concept of mini-circles. I am wondering what would be the experience of someone who used them, or any practical...

03 April 2017 8,177 2 View

What would be the difference if I use the Web server instead of dedicated server for protein modeling and docking?

We know that software tools like I-tasser etc have a web server and a standalone option too. Is there any difference to protein C-scores or efficiency or accuracy if you do it on standalone...

03 April 2017 6,520 0 View

Can anyone suggest me the best computational tool for predicting antigen antibody affinity?

I have modeled the 3D structures of the antibody and the antigen, now I have several antibodies against the same antigen, I am looking for tools that would tell me which antibody has the maximum...

02 March 2017 1,618 3 View

Which is more reliable, I-Tasser or Rosetta?

I am currently trying to model my proteins (they are antibody fragments) I am looking for this comparison between the two major tools for protein model prediction. I either find people saying to...

02 March 2017 8,615 9 View

Gaussia luciferase at N terminus or C terminus of the protein? Which is better?

what are advantages/disadvantages of adding gaussia luciferase on N terminus than on C terminus of the protein of interest. and would it work if I attach the gluc signal peptide at the N- terminus...

02 March 2017 3,971 1 View

Can I use ethanol and glycogen to concentrate my RNA sample??

I have 10^5 cell mammalian culture and I have isolated total RNA from them, as a part of an important experiment, however the yield was very low. Though I have more than required amount, the...

03 April 2016 2,509 6 View

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