what are advantages/disadvantages of adding gaussia luciferase on N terminus than on C terminus of the protein of interest.
and would it work if I attach the gluc signal peptide at the N- terminus of the protein of interest and leave the gluciferase on the C- terminus of the protein of interest?
Yuan-Yeu Yau
To be safe, you should build both constructs and test them out. It is a matter of protein folding. Some work well when it attach to the N-terminus of your protein-of-interest, some work well when it attach to the C-terminus.
See a similar discussion on RG:
.https://www.researchgate.net/post/What_is_the_difference_in_tagging_GFP_at_the_N-terminus_or_C-terminus
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