I was looking at some single cell RNA Sequencing data and I was wondering how researchers determine certain cell types for example what differentiates M1 vs M2 macrophage in a data set. What genes should I be looking for? I've looked through many resources and most only describe cell surface markers that I could look for which don't actually show up as highly expressed in my sc seq data. Any advice or database would really be helpful! Thanks.
Hello Sammy Shi
The following two manuscripts might be helpful to take your decision.
Dear Sammy Shi
please check the attached publication.
Article Novel Markers to Delineate Murine M1 and M2 Macrophages
All the best & good luck with your experiments,
Michael
There are several articles that describe the markers. This is one good article that describes the markers for M1 and M2:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3944738/pdf/biolrep-06-13.pdf
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