I am conducting an experiment where I would like to detect inflammatory mouse proteins and measure its concentration in SDS. Does anyone have experience on this issue? The method based in flow citometry is efficient for protein measures? Thanks.
I have used the Mouse Inflammation Kit, that contents reagents for capture and detection of IL-6, IL-10, IFN-γ, TNF, and IL-12p70. Which tissue you will use to quantitate the proteins?
If you are looking for serum cytokine levels I would use a cytokine multiplex kit or a precoated Elisa kit with multiple cytokines ( the more cytokines, the higher the price). Alternatively, you could just do qpcr on the selected tissue, this much more affordable.