How can i redraw the published figures to reproduce them in my articles?

is there an application or website that can be used to redraw and edit the figures to re-publish them without permission from the main author?

21 December 2020 921 3 View

Shall i use the country's CPI score or rank while entering the data ?

I have the Corruption perception index as a control variable in my study and i'm not sure what i have use the score or the rank of the country ? please advice what i have to use in the data entry...

09 December 2020 3,797 2 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View

How can I prepare a stock solution from alcoholic extract of peganum harmala seed ? And how prepare multiple concentration ?

I used this method to make concentrations, by using distilled water to make concentration like 25mg/ml 25mg of extract in 1ml of distilled water and so on ....

29 November 2020 2,933 4 View

Cell culture Vitamin D free media ???

Dear researchers, I am working onn the influence of vitamin D on cancer. I was wondering whether ther is Vitamin D free media , since I wanna be completly sure of my negative control without...

01 November 2020 582 2 View

What does R^2 value mean?

Hello, -So I have a set of bacterial communities extraced from rhizospheric soil in both saline and control environments for two different cultivars of plants, one is tolerant to salinity and the...

07 May 2020 5,750 4 View

Western blot , Blocking step milk vs BSA ?

concerning the westrn blot ..........I am diluting my antibodies in 5% bsa , but I get very high backgroud though I an diluting the secondry antibody in 5% milk . My question is . Can I block the...

13 April 2020 297 4 View

If I want to know the full length of a specific gene and the ORF of this gene, should I do RACE PCR or there is another way?

If I want to know the full length of a specific gene and the ORF of this gene, should I do RACE PCR or there is another way? Note that the whole genome of the insect is published.

23 January 2020 2,377 1 View

Unidentified peak in proton and C13 NMR using DMSO-d6 or MeOD as solvent?

The NMR of a series of novel compounds synthesized using identical procedure on different time intervals -all- showed a strong single peak at around 8.46 in proton NMR using DMSO-d6 or MeOD, the...

22 October 2019 6,285 2 View

How can I determine the optimal concentration of the plant extract(Pistacia atlantica) added to meat???

which is at this concentration reduction bacterial growth is less than possible ?? and for extending the shelf life of minced meat

12 June 2019 8,486 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View