Can't generate a mash for topology in ANSYS. how to do it?

Created model of steel caisson in space claim. Used a static structure for topology. But there's an error "one or more surfaces cannot be mashed with acceptable quality", " One or more entities...

25 January 2021 893 1 View

Do you know any country-specific methodologies of urban public spaces evaluation?

Hello! Currently I am working on a research about different methodologies of urban public spaces evaluation/their impact assessment. The only comprehensive projects with explicit measurement...

18 November 2020 2,428 5 View

How to understand question about epistemological starting points and theory on one side and method on the other?

The whole question looks like that: Discuss the relation between epistemological starting points and theory on one side and method on the other, concerning two methods (or two methodological...

31 October 2020 2,580 6 View

Alternate cell viability assay for MCF7 cells?

Hi All, I've been treating several cancer cell lines and a non-cancer cell line with WST-1, a variation of the MTT or MST viability assays, to monitor cell death with my immunotherapy drug. All...

10 March 2020 2,415 10 View

EPR: What to do if there is no hyperfine?

What are the most common factors besides clustering and spin-spin interactions of paramagnetic atoms that lead to poor spectra without expected hyperfine?

19 March 2019 9,885 2 View

Is there a way to coat latex beads with a ROS scavenger such as sodium pyruvate?

Does anyone know if it is it possible to coat 1um latex beads with a ROS scavenger such as sodium pyruvate? Do the beads need to be modified? Thank you!

11 March 2018 6,726 3 View

Dye front is separating into a "blob" - blue on top, purple on bottom?

I'm attempting to probe for low molecular weight proteins (11-25 kDa) from whole-cell lysates using a Tris based RIPA lysis buffer with inhibitors. I've been trying to separate these extracts...

03 December 2017 2,581 5 View

Liposome delivery

Hello everyone, I am looking to see if it is possible to encapsulate soluble bacterial peptidoglycan in a liposome. Are there any kits out there I could use? They seem to be mostly for DNA/RNA...

17 May 2017 4,967 3 View

Does anyone know a method (or commercially available kit) for simultaneous isolation of plasmid DNA and total RNA from the same sample?

28 August 2016 2,145 3 View

What is the history of pop-up areas? Is there are any theoretical information?

I need to know more about history of pop up places (shops, restaurant and so on), but from the theoretical side

18 May 2016 9,297 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View