Can't generate a mash for topology in ANSYS. how to do it?

Created model of steel caisson in space claim. Used a static structure for topology. But there's an error "one or more surfaces cannot be mashed with acceptable quality", " One or more entities...

25 January 2021 893 1 View

Do you know any country-specific methodologies of urban public spaces evaluation?

Hello! Currently I am working on a research about different methodologies of urban public spaces evaluation/their impact assessment. The only comprehensive projects with explicit measurement...

18 November 2020 2,428 5 View

How to understand question about epistemological starting points and theory on one side and method on the other?

The whole question looks like that: Discuss the relation between epistemological starting points and theory on one side and method on the other, concerning two methods (or two methodological...

31 October 2020 2,580 6 View

EPR: What to do if there is no hyperfine?

What are the most common factors besides clustering and spin-spin interactions of paramagnetic atoms that lead to poor spectra without expected hyperfine?

19 March 2019 9,885 2 View

Is there a way to coat latex beads with a ROS scavenger such as sodium pyruvate?

Does anyone know if it is it possible to coat 1um latex beads with a ROS scavenger such as sodium pyruvate? Do the beads need to be modified? Thank you!

11 March 2018 6,726 3 View

Liposome delivery

Hello everyone, I am looking to see if it is possible to encapsulate soluble bacterial peptidoglycan in a liposome. Are there any kits out there I could use? They seem to be mostly for DNA/RNA...

17 May 2017 4,967 3 View

What analysis and what software should I use if I want to model how one censored variable predicts another censored variable?

My research has to do with employment and reincarceration after release. I have used cox proportional hazard model to see whether participating in vocational education while in prison affects the...

25 January 2017 8,624 2 View

What is the history of pop-up areas? Is there are any theoretical information?

I need to know more about history of pop up places (shops, restaurant and so on), but from the theoretical side

18 May 2016 9,297 2 View

Why do my cell not adhere to the culture dish after?

22 December 2014 4,513 10 View

Can I change TaqMan probe dyes?

I want to detect 50-bp segment of the recA gene Bordetella avium. Seqence of probe and primers: 5-CATCGCGCTGGGTG-3 NED fluorescent reporter on the 5' end and nonfluorescent black hole quencher...

26 January 2014 2,064 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View