Does anyone know a method (or commercially available kit) for simultaneous isolation of plasmid DNA and total RNA from the same sample?

More Daria Onichtchouk's questions See All

Geisser-Greenhouse approximation underestimating significance?

I have performed a fairly standard kinetics experiment, in which I investigated the stability of various oligonucleotides in an enzyme solution, by incubating the oligonucleotides with the enzyme...

17 April 2024 3,655 8 View

Are there reserved variables in CST Studio Suite 2021?

Hello! Are there reserved variables in CST, for example, the speed of light, mass and charge of an electron? What value for the speed of light is used in CST?

31 October 2023 8,476 2 View

Precipitate in Influenza control antigen from IRR. Is it OK?

Hello everyone! We received Influenza B Control Antigens BPL inactivated for HAI. It looks like slightly yellow transparent fluid with white flakes. I can't find any information on IRR website,...

08 August 2023 9,500 0 View

Effect of NaCl concentration on a 23Na pulse length?

It is known that sample conductivity affects pulse durations in 1H NMR experiments. But how does the concentration of NaCl in a sample affects pulse durations in sodium-23 (23Na) NMR experiments?...

18 June 2023 1,708 4 View

What post-hoc test should I use for nominal variables, after chi square test?

Hello! I would like to address the experts regarding a question about conducting a statistical analysis using only nominal variables. Specifically, I would like to compare the responses of survey...

01 April 2023 3,688 4 View

Alternative for syncytium formation assay?

I want to test whether my anti-HA monoclonal antibodies can inhibit the membrane fusion step of influenza virus entry, but we don't have any resources for syncytium formation assay with...

07 February 2023 2,731 0 View

Url defense blocking access to papers sent by request?

Please explain what is going on with this platform? Authors send me links to full papers and a url defense pops up. At this site, the papers remain inaccessible. It is impossible to request...

13 December 2022 9,525 0 View

Mycobacteriophage plaque assay procedure?

Hello, I have a question about double layer agar method for plaque assay for mycobacterium. Why my bacterial lawn of Mycobacterium smegmatis is not homogeneous? What could be the reason of growth...

02 November 2022 7,925 0 View

Any ideas how to quench autofluorescence in mammalian live cells for confocal?

Hey everyone! Have anyone faced an issue of how to minimize/quench autofluorescence in mammalian live cells? To be more specific I always stained and visualized ovarian cancer cell lines in blue,...

17 February 2022 2,492 0 View

Does anyone have experience in solubilization plasma membrane with SMA polymer?

Hey everyone! Would be really appreciated for advice. Trying to solubilize plasma membrane with Styrene - maleic acid (SMA) copolymers. Following all the instructions making a 5% SMA solution,...

04 August 2021 5,572 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Gael Lecellier

Hi,

I can recommand the RNA/DNA shield (https://www.zymoresearch.com/rna/rna-stabilization-preservation/dna-rna-shield) which works fine.

Daniel Gómez-Cabello

Any protocol using TriReagent or TriZol. You can obtain DNA, RNA and also proteins for the same sample.

Ariadne Hernandez

Hello, Fenol cloroform methods (commercial brands: Trizol, DNAzol, etc.)