Hello everyone,
I am looking to see if it is possible to encapsulate soluble bacterial peptidoglycan in a liposome. Are there any kits out there I could use? They seem to be mostly for DNA/RNA delivery .
Thank you in advance for your anwsers!
You could probably use the same dehydration-rehydration technique that has been used to encapsulate other large molecules.
http://www.nature.com/nbt/journal/v2/n11/abs/nbt1184-979.html
Dear Daria, I recommend you to pay attention the following link. Maybe it will be helpfull for your studies.
Best regards.
https://www.precisionnanosystems.com/formulation-development/polymeric-nanoparticles/
During my work with membrane transport proteins, I had to prepare substrate preloaded proteoliposomes for antiport measurements.
For this, I simply resolved 10% (m/v) phosphatidyl choline in my buffer containing the substrate I wanted to encapsulate and then sonicated it for 10 minutes with a tip-sonicator (20 kHz, 60 W amplitude, 50% duty cycle) while the stuff being cooled in an ice bath.
But after sonication, the liposomes have to be separated from the free substrate, which might be the tricky part. We were able to use DOWEX spin columns that would bind the ADP we used, but I guess it's a bit more difficult for peptidoglycan. Technically you can use centrifugation at high g's to collect the liposomes, the SUV's should mostly survive that.
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