Is there a replacement for using 70% Formic acid when extracting Amyloid Beta from Cell culture?

I came across some protocols using Formic Acid in the first step of Amyloid beta extraction from cultured cells. Formic Acid is not the safest substance to use, so I was wondering if there is a...

09 September 2019 5,845 1 View

What measures of risk-assessment behavior in mice do you perform?

Hello, In a study we perform, risk-assessment is a central behavior. Our available mazes are the Open Field, Dark Light test and Elavated Plus Maze. So far we perfromed protected and unprotected...

09 June 2019 4,577 2 View

What should be the "my plasmid"/GFP plasmid ratio in co-transfection?

Hi, I would like to co-transfect U2OS cells with GFP-containing plasmid, and my plasmid of interest using Polyjet transfection reagent. The main idea is to measure trasnfection efficiency, and...

17 March 2017 6,307 1 View

Cloudiness after adding isopropanol in RNA isolation using TRI-reagent. Why does it happen?

22 February 2017 2,135 5 View

Can Zipf law exist in a canonical ensemble?

Zipf law (which is a power law) is the maximum entropy distribution of a system of P particles in N boxes where P>>N. Its derivation is based on microcanonical ensemble in which the entropy is...

20 May 2014 8,674 3 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to increase mesh quality of a cfd model in ansys fluent?

How can one improve mesh skewness, orthogonality, aspect ratio?

28 February 2021 7,730 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

How to find a correlation between coordinates in a curvilinear coordinate system?

Hello. I have a skewed elliptical coordinate system (I use alternative elliptical coordinates). And I need to determine connections between coordinates in order to fetch a covariance matrix of...

28 February 2021 364 3 View

Why is my CFD problem (mini heat sink rectangular channel) not converging while having good quality mesh?

My CFD problem is having two interconnected rectangular channel, which is known as mini heat sink. I have already performed parallel flow inside of an interconnected mini heat sink. However, I...

19 February 2021 3,209 3 View

How to compare means of continuous data with zero-inflation and skew?

I have two groups (unpaired) of plant biomass measurements and want to know whether there is a test that can be used to determine statistical significance where assumptions are not violated. The...

18 February 2021 1,258 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Is my GFAP staining in right position?

I was differentiate hESC to astrocyte. In the second week of differentiation, I stained my cell with s100beta and GFAP. I expected that the staining site of GFAP would come from the cytoplasm,...

15 February 2021 8,982 1 View

Does anybody know the ways of homogeneous ripening of Mung bean crop?

Some crops (Mung bean) are matured at different times i.e., harvested at several times that's why the labour cost of the farmer is high. So, could you please, is there any ways or chemicals are...

12 February 2021 294 4 View