Is there a replacement for using 70% Formic acid when extracting Amyloid Beta from Cell culture?

10 September 2019 1 6K Report

I came across some protocols using Formic Acid in the first step of Amyloid beta extraction from cultured cells.

Formic Acid is not the safest substance to use, so I was wondering if there is a proven safer protocol that can be used to extract Amyloid Beta (both soluble and insoluble) from cultured cells.

I am interested in extracting it from Astrocytes.

Thanks for any advice.

Bahman Sadeghi

Yes! Most of the protocols use formic acid as a component at the purification stage like the following famous one:

https://www.nature.com/articles/s41598-017-10422-x

But check the following older papers. Maybe you can find some useful methods without that stage:

http://www.jbc.org/content/267/24/17082.short

http://www.jbc.org/content/267/4/2214.short

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