What additional supplements are required for THP1 cell culturing?

18 August 2017 0 8K Report

I had tried culturing THP1 cells in RPMI-1640 medium supplemented with 10% FBS and 1% antibiotic-antimycotic cells but still, the cells didn't attach to the surface of flask after 3 days.

Are there some additional factors that have to be used in its passaging or subculturing?

Any views will be highly appreciated.

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