Rohit Kumar Tiwari

6 Questions 8 Answers 0 Followers

Questions related from Rohit Kumar Tiwari

How to characterize epitopes within a peptide sequence?

Greetings In a theoretical perspective, I want to use a human-derived peptide as a plausible antigen but the problem associated with tolerance hampers my approach. Then I thought why not denature...

21 January 2021 2,380 1 View

Coupling of proteins with polysaccharide derived nanoparticles?

I am wanting to couple my whole cell lysate with polycation coated nanoparticles but really confused !!! Can anyone provide with some insight. Thanks in advance

08 September 2017 5,948 5 View

How to proceed for XRD of nanoparticles dispersed in non-volatile solvents like water?

I have my polysaccharide derived nanoparticles dispersed in water. I am interested in doing XRD analysis of my sample but haven't done this analysis before. Can anybody give me an insight how can...

31 May 2017 2,641 4 View

Concentration of CBA flex set capture beads and IFN-gamma antibodies to be used?

I am trying to detect the IFN-gamma levels (quantitative analysis) in J774 cells treated with specific polysaccharides using CBA Flex set from BD Biosciences. the kit does not tells about the...

08 February 2017 5,018 7 View

How to make nanoparticles less than 10nm form Beta-glucan polymers?

the nanoparticles need to be internalized into cell line J774.

02 June 2016 8,642 5 View

If the concentration of the DNA sample being used is 2micromolar and I want the adjust the ratio to 10?

concentration of the DNA in sample is 2micromolar the N/P ratio has to be fixed to 10.i have calculated that the concentration of the polycation has to be 93.8nanomoles. Is this right? any help...

01 January 1970 3,131 1 View