We used primer with 163 pb and another with 754 pb for the same microbe ,which one prefer and which one correct ?

More Qassim Kshash's questions See All

Please ,any suggestion about increse RBC fragility after exposure lab animals into artificial light 24 hrs?

Lab experiment

02 March 2018 8,677 8 View

What are the specific immunological marker available as kit in lab animals ?

lab expermint

02 March 2018 5,000 7 View

What is the standard pH in the oral cavity that does not allow tooth decay?

Tooth Decay

31 December 2017 3,245 18 View

If the success rate for your students is high or vice versa, does that reflect?

If the success rate for your students is high or vice versa, does that reflect 1- The intellectual maturity of your students 2 - your teaching competence 3 - Ease of questions 4 - students love...

31 December 2017 4,892 20 View

Do you think the level of education is developing or declining in your country?

Education as general

10 November 2017 2,266 22 View

What are the role of universities and graduates in the third world countries ?

In the third world countries there are universities and colleges and a huge number of graduates in all disciplines and there are raw materials and human resources, to see any impact of...

10 November 2017 3,063 13 View

How we can do brain stimulation in depression case?

Brain

09 October 2017 8,097 9 View

What is about the hard-RAT AVAT?

I WANT TO LEARN ABOUT THAT

05 June 2017 1,371 6 View

Which lab animals is prefer to prepare antibodies?

Antibody

04 May 2017 4,720 6 View

Are some indicators in preparation media affected by autoclave or not like phenol red?

Indicators

04 May 2017 9,257 15 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How do you delete a duplicate pdf for the same paper on ResearchGate?

The first pdf file I uploaded had an error. So I uploaded an updated, corrected pdf of that paper with a different pdf name. I dpon't want the old copy to be download or read.

07 August 2024 9,508 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

What is the ionic radius of Hydrogen anion? And how to know the exact ionic radii of the elements?

I'm trying to calculate the tolerance factors of the hydride perovskite and the value calculated by me and the reported values are contradictory. Need more clarity in using the correct ionic radii...

01 August 2024 2,614 1 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

What role do microorganisms play in nutrient cycling and maintaining soil health in nature and role of microorganisms in the cycling of bio elements?

31 July 2024 3,965 0 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

How do pollutants in soil affect soil microorganisms and role do microorganisms play in nutrient cycling and soil fertility?

31 July 2024 368 2 View

What's biological importance of bacteria &role of microbes improve crop productivity &soil health &role of bacteria as decomposers incycling nutrient?

What are the biological importance of bacteria and the role of microbes to improve crop productivity and soil health and role of bacteria as decomposers in cycling nutrients?

31 July 2024 3,479 5 View