We used primer with 163 pb and another with 754 pb for the same microbe ,which one prefer and which one correct ?

More Qassim Kshash's questions See All

Please ,any suggestion about increse RBC fragility after exposure lab animals into artificial light 24 hrs?

Lab experiment

02 March 2018 8,527 8 View

What are the specific immunological marker available as kit in lab animals ?

lab expermint

02 March 2018 4,836 7 View

What is the standard pH in the oral cavity that does not allow tooth decay?

Tooth Decay

31 December 2017 3,093 18 View

If the success rate for your students is high or vice versa, does that reflect?

If the success rate for your students is high or vice versa, does that reflect 1- The intellectual maturity of your students 2 - your teaching competence 3 - Ease of questions 4 - students love...

31 December 2017 4,747 20 View

Do you think the level of education is developing or declining in your country?

Education as general

10 November 2017 2,108 22 View

What are the role of universities and graduates in the third world countries ?

In the third world countries there are universities and colleges and a huge number of graduates in all disciplines and there are raw materials and human resources, to see any impact of...

10 November 2017 2,902 13 View

How we can do brain stimulation in depression case?

Brain

09 October 2017 7,946 9 View

What is about the hard-RAT AVAT?

I WANT TO LEARN ABOUT THAT

05 June 2017 1,222 6 View

Which lab animals is prefer to prepare antibodies?

Antibody

04 May 2017 4,569 6 View

Which samples(serum, urine, aqueous humor, CSF) are prefered in PCR detection of leptospira?

Leptospirosis

04 May 2017 171 17 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View