Isolated ovaries can be kept in culture in two ways depending on the age of the donor. If the ovary is from a newborn animal (rodent) it can be kept in culture using a floating disc. There are many publications using this method but one example is described in Tuttle et al., 2009 Hum Reprod. 6:1452-9. Now, if you are trying to study functional characteristics of the whole ovary in culture then you are forced to use a much more complicated method of whole ovary perfusion and maintenance in culture. For details of the method see Jarrell et al. 1993 Reproductive Toxicology 7 Suppl 1:63-8. This is a review paper that will lead you to several of Dr. Jarrell's publications that detail the methods and provide insight into useful outcome measures.

IS IT EMBRYO CULTURE OR ORGAN CULTURE?

Organ culture.