I have no experience on both product.

But, in our lab, Promega TNT T7 insect protein expression system has been used and successfully got relatively good crop.

No comparison, not sure which one is better.

Thanks. But did it work with a regular expression vector with a T7 promoter, or did you have to reclone into their "optimized" vector?

We ligated vector (w/ T7 promoter) into KpnI site of pTNT vector.

I ever used pGBKT7 and pGADT7 both are T7 promoters, the kit Promega TNT, which can give nice protein expression for CO-IP

Sounds good. But did you use the insect cell extract or the reticulocyte lysate TnT kit?

I expressed protein with reticulocyte lysate (Promega) using pTNT vector.

It's pretty simple, mixture, 37 degree incubation for 60 to 90 min.

the reticulocyte lysate TnT kit I used from Promega

good luck

Also if you don't want to reclone your gene but are ready to do a PCR, you can PCR your gene with the t7 promoter sequence in one of the primers (no need if your gene is already cloned downstream of a t7 promoter of course). You simply use the PCR product to do your in vitro transcription/translation