Olivier Kassel

4 Questions 43 Answers 0 Followers

Questions related from Olivier Kassel

pcDEF3 vector sequence

Does anyone know where I could find the sequence of the pcDEF3 expression vector?

11 November 2012 5,026 8 View

Vector for in vitro cell-free protein expression

Has anyone successfully used pcDNA3 as a template to express a protein using either the Thermo Scientific 1-Step Human Coupled IVT Kit – DNA or the Promega TNT T7 Insect Cell Extract Protein...

10 October 2012 3,356 8 View

DNA quantification

Hi everybody. Does anyone know of a fluorescent dye (or another method) to quantify total DNA from a whole cell lysate (in RIPA)?

01 January 2010 2,123 3 View

HeLa electroporation

Hi, Does anyone have a GOOD electroporation protocol for HeLa cells? I mean giving at least 90% transfection efficiency? I don't mind killing 50% of the cells if the remaining cells are all...

01 January 2010 10,075 3 View