The clones obtained from embryos (grown in virtro) of douglas fir are significantly differents although they were derived from somatic embryogenesis and grown in the same conditions??
Does anyone explain that aspect?? (see attached figure (roots and aerial part!!!))
Dear Mohammad
Sometimes we see the someclonal variation in tissue culture. Has embryogenesis been obtained from callus? What plant growth regulators have you used?
Embryos were obtained from primary cotyledonary embryos subjected to second embryogenesis. PGR used were 2,4-D and BA.
Probably the production of secondary embryogenesis and the use of 2,4-D have caused these abnormalities.
It is due to somaclonal variations primarily. Other factors are growth regulators, cultural conditions etc
Somatic embryogenesis can take place either direct (from the explant itself, like cotyledons, leaves etc.) or indirect (via an intermediate callus stage) mode. If it is the first case, the chance of maintenance of clonal fidelity is more. In spite of the plants are genetic clones, plants often look different in the initial stage, but after attaining proper maturation, they become 'true-to-type'. My suggestion to the authors to try some molecular markers for this purpose to ascertain it simultaneously. Plenty papers are available on this aspect on different plants, and I suppose for douglas fir also.
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