Papers of interest :
Chapter History, Discovery, and Classification of lncRNAs
Article From Discovery to Function: The Expanding Roles of Long Non-...
Wilusz et al. Long noncoding RNAs: functional surprises from the RNA world, Genes Dev. 2009. 23: 1494-1504. PMID: 19571179
Bhartiya et al. Conceptual approaches for lncRNA drug discovery and future strategies. Expert Opin Drug Discov. 2012. 7: 503-513. PMID: 22559214
Apparently most strategies are more towards inhibiting LncRNAs instead of using them directly.
Might want to consider the vectors of such and the possible interferon response if those are first transcribed as dsRNA.
Depending on how broad you want to go in your question about non-coding RNAs and the regulation of gene expression, you'll want to become familiar with some of the older terminology.
Plant scientists called this "quelling" or "quenching" of a phenotype. Classic example is in the flower pigment pathway in petunias.
C. elegans scientists called it "silencing". See the Timmons & Fire paper for RNAi in C. elegans.
They still argue over who "discovered" gene silencing first.
Thank you Mrs. Katie A Burnette for your answer.
I know that gene silencing is mediated by siRNA or miRNA at transcriptional or post-transcriptional levels but what about lnRNA?
I might suggest into overexpression of lncRNA on PubMed (NCBI not working for me right now). Also, you might to look at the use of lncRNA as microRNA sponges. I stay around, interesting topic.
Mohammad Issawi - there are lots of recent publications on long non-coding DNAs in gene silencing in many systems (human, cancer cells, plants, etc.)
Classic example is Xist in X chromosome silencing. A Google Scholar search will pull up thousands of article, I'm sure you'll find plenty of references to answer your question.
Best,
Dr. Burnette
Thank you all for your valuable contributions.
So it is such of interest how LnRNAs are acting as miRNA sponge (unsilencer) or in contrast, as gene silencer in embryonic development like X inactivation and neuronal differenciation!!!
It seems that this double capacity of lnRNAs still remains elusive and far from being elucidated!
There are several reports regarding gene silencing action mediated by non-coding RNAs....
here are such papers of interest
https://www.nature.com/articles/ncomms10406
https://www.thermofisher.com/in/en/home/life-science/rnai/synthetic-rnai-analysis/ambion-silencer-select-sirnas/make-your-silencing-roar.html
Article Non-coding RNAs as regulators of gene expression and epigenetics
Article Gene regulation by the act of long non-coding RNA transcription
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