How to normalise non-parametric data that remains non-parametric after log transforming and SQRT the data?

I am looking at blood lactate values after completing fold change on the data set. I have 3 testing sessions with pre and post measures in each case. When testing for normality the data shows some...

16 October 2019 3,186 4 View

How do I analyze repeated measures data, with 5 treatments, but each treatment has a different temporal scale?

Thanks for reading. This is my experimental design 1) 5 treatments containing 15 individuals each (75 individuals total) 2) Variable Y is measured over time, however this time-scale is different...

31 January 2018 6,485 5 View

How cladism was perceived in former Soviet Union, and how is it perceived now in Russia and other eastern European countries?

Since cladistics and then cladism were born in Western Germany and then developped in English-speaking Western countries, I am interested in the perception of this classificatory philosophy in the...

15 May 2017 6,011 22 View

Shouldn't the agent of Canine Transmissible Venereal Tumor be considered a full-fledged species?

27 July 2016 2,897 3 View

Any tips for intra-cellular cytokine staining in granulocytes?

I have been trying to look for intra-cellular TNFa and IL-12 in granulocytes. For this, I have used Golgi-Plug (Brefeldin A) to block cytokine release. Unfortunately, it looks like either the...

31 May 2016 1,073 3 View

Is some research involved in silver eel catch and transport downstream powerhouse available for some share of information?

14 December 2015 6,832 5 View

Will ligase work on DNA strands broken by heat?

After heat shattering a DNA strand it into smaller strands (not denaturation), what kind of ends are produced? Are these able to be ligated?Thanks!

01 August 2015 1,995 4 View

Does anyone know where to order custom rabbit monoclonal antibody?

It is for double staining immunofluorescence and my other antibody is a mouse monoclonal antibody. I plan to use Alexa goat IgG conjugates as secondary antibodies. Molecular probes do not provide...

10 June 2013 853 7 View

Database of thermal and optical properties of construction materials

Hello everybody, could you help me please to found reference database (or research articles) about thermal and optical properties (absorbance, reflectance, transmittance and thermal...

01 January 1970 7,778 6 View

Warm spot competition test to assess social hierarchy in mice ?

I would like to adapt the warm spot competition test to assess the social hierarchy in groups of mice. This assay has been described in the Zhou et al., 2017 in Science (Paper attached: History of...

01 January 1970 573 1 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View