Hamed Moslehi

2 Questions 9 Answers 0 Followers

Questions related from Hamed Moslehi

Is spacer nucleotide between RBS and start codon effect on protein experssion?

Hi everyone I inserted a restriction site (5 nucleotides) between RBS and the start codon (these 5 nucleotides are upstream of multiple cloning site). after confirming my cloning, we started...

23 July 2023 1,340 5 View

How predict of expression recombinant protein?

Hi, does anyone know of a database or method for predicting the expression of a recombinant protein in E. Coli which strain of E. coli is better to have the highest soluble expression of the...

05 July 2023 2,572 4 View