Hamed Moslehi

6 Questions 14 Answers 0 Followers

Questions related from Hamed Moslehi

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

22 July 2024 8,638 1 View

Is difference between co-transformation and chemical competent?

Hi everyone I have 2 vectors and I want to transform these into one bacteria cell (SHuffle strain). Do you think co-transformation (Transforming 2 vectors together in one step into the competent...

30 August 2023 4,035 4 View

Is spacer nucleotide between RBS and start codon effect on protein experssion?

Hi everyone I inserted a restriction site (5 nucleotides) between RBS and the start codon (these 5 nucleotides are upstream of multiple cloning site). after confirming my cloning, we started...

23 July 2023 1,346 5 View

How is Triple ligation protocol?

Hi everyone I have 3 fragments of digested plasmids with length of 2400,1500 and 200 bp (with compatible sticky ends). It would be great if you could advice me how to ligate these three DNA...

20 July 2023 4,279 4 View

How predict of expression recombinant protein?

Hi, does anyone know of a database or method for predicting the expression of a recombinant protein in E. Coli which strain of E. coli is better to have the highest soluble expression of the...

05 July 2023 2,578 4 View

lack of protein expression by bacteria and /or vector

Hi I am currently studying the expression of the TEV protease recombinant protein, and unfortunately, I have encountered a somewhat illogical problem during my work. I would greatly appreciate it...

01 January 1970 5,958 4 View