Damien Bretagne

2 Questions 2 Answers 0 Followers

Questions related from Damien Bretagne

Utilization of pRSET plasmid into BL21(DE3) for protein expression?

Hello everyone, I would like to know if someone already used the pRSET plasmid into normal BL21(DE3) and not into BL21(DE3)pLysS for protein expression. I'm having some induction and purification...

04 July 2023 6,852 3 View

How resolve induction problem pRSET plasmid into BL21 (DE3)?

Hello everyone, I'm currently tryning to overexpressed and purified a protein from saccharomyces cerevisiea into BL21 (DE3). I'm currently using the pRSET plasmid and normal BL21 (DE3) However,...

16 June 2023 7,460 2 View