What is the best telemetry device for wireless monitoring of temperature in mice ?

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What are exactly the different types of fetomaternal interdigitations in placentas?

I am quite confused because some sources distinguish between 5 types: folded, lamellar, villous, trabecular, labyrinthine (e.g. Benirschke's Pathology of the Human Placenta ; Evolution of the...

13 March 2024 7,798 2 View

Utilization of pRSET plasmid into BL21(DE3) for protein expression?

Hello everyone, I would like to know if someone already used the pRSET plasmid into normal BL21(DE3) and not into BL21(DE3)pLysS for protein expression. I'm having some induction and purification...

03 July 2023 6,853 3 View

How resolve induction problem pRSET plasmid into BL21 (DE3)?

Hello everyone, I'm currently tryning to overexpressed and purified a protein from saccharomyces cerevisiea into BL21 (DE3). I'm currently using the pRSET plasmid and normal BL21 (DE3) However,...

15 June 2023 7,461 2 View

SQUID output impedance regarding the bias, vs, dynamic output impedance ?

A SQUID (Superconducting QUantum Interference Device) can be used as a magnetic flux to voltage converter. A DC current biasing I_SQ=I_0 is usually apply. Looking at the I_SQ(V_SQ, Flux) output...

28 August 2022 5,622 0 View

Latent profile analysis with tidyLPA : issue with means ?

Hello everyone, I run a Latent Profile Analysis in R with the package tidyLPA to identify groups of participants who showed similar patterns on burnout variables (3 variables : exhaustion, cynism...

08 December 2021 4,630 2 View

Cell culture contamination?

Is this contamination or cell clumping? its a flask of hek 293 cells 24hrs after passaging. no clour change or turbidity of media.

28 September 2021 5,936 5 View

Which software is used for electric network simulation ?

hello. Could anyone suggest a software (in open access if possible) capable to simulate and identify electric network problems in interconnecting building systems. Thanks in advance.

02 July 2021 9,724 8 View

How to measures the spectral noise density in V/√Hz from a modern spectrum analyser providing power in dBm ?

We are using a R&S FSV3004 spectrum analyser. As many modern spectrum analyser it measures in dBm. To try an harmonic measurement of noise (noramlized per 1 Hz frequency unit) we have put a 10...

13 December 2020 7,300 0 View

Self restoring logic to correct single event upsets in a radiation hardened by design ASIC with no need of clock?

Application-specific integrated circuit (ASIC) designs for space applications must address radiation environments that can changes stored values in registers. Indeed, high-energy particles can set...

23 June 2020 5,952 0 View

What is the Impedance of an IC (integrated circuit) bond pads and its associated wire bonding for MHz application?

I hear that 100µm square PADs commonly used for VLSI design introduces a typical capacitance of about 5 pF (for sure it depends to many specific cases of packages). And a wire bonding used for...

11 May 2020 8,800 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,708 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,122 4 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

How can I get clearer background for my western blot?

I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...

29 July 2024 6,483 5 View