The use of Trabedectin has been found effective in the treatment of ovarian cancer, and other soft tissue cancer. Could it also be effective in the treatment of non-small cell lung cancer?
It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal
29 November 2020 2,554 3 View
Hello everybody I've been trying to seed microglia from adult mice and I haven't been successful. I isolated them using Miltenyi Beads and that worked perfectly, passed them through the...
14 October 2020 4,572 3 View
Hi everyone Im trying to slice my mice brains with the cryostat, however, I haven't been successful, the tissue seems to stretch and then it breaks. Let me tell you the story about these brains:...
25 August 2020 6,479 4 View
Hi everyone, I am trying to transfect murine CD4+ T cells in vitro, using Lipofectamine RNAi/MAX however, even when trying troubleshoot, the positive FITC labelled control shows just about 3%...
03 June 2020 2,618 1 View
Hello all, I am running a Geographically Weighted Regression and calibrating the bandwidth of my models using GW.model R functions. After some tests and playing around with the data, I...
16 May 2019 3,652 1 View
Hello, I need your help. We are studying electrocardiographic parameters in an experimental model of heart injury, and we obtained unexpected results. The ECG intervals alternated during the...
28 September 2017 2,581 4 View
Good day, I try to make a pcr nested but I have problems of contamination, I use as a template the negative product of the pcr in the second pcr and gives me positive. I thought that the pollution...
24 January 2017 6,992 2 View
Hi guys, does anybody know any CRH antibody which would work for western blots giving a nice and specific signal, especially in brain tissues (PVN, hippocampus)? Any advice is welcome. Thank you.
27 January 2016 320 3 View
Hi guys! I'm sequencing and assembling a de novo invertebrate genome of about 0,8Gb. I want to make a lane run of Illumina nextera mate-pairs, but I'm having some difficulty on selecting the size...
06 January 2015 6,134 9 View
Thanks in advance.
22 February 2014 7,262 4 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...
02 March 2021 8,149 3 View
Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?
01 March 2021 8,087 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?
23 February 2021 3,840 3 View
Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...
21 February 2021 8,945 3 View
I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...
20 February 2021 6,879 3 View
The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...
16 February 2021 1,762 8 View
I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...
14 February 2021 3,142 7 View