What are the cost effective ways to identify and isolate true cancer stem cells?

There are many methods for identifying and isolating breast CSCs but they are quite expensive. Does anyone have any alternate idea for isolating true CSCs? I want to isolate it from patient tissue...

07 August 2016 2,758 16 View

How to understand the behaviour of SF9 cells?

I am new to the insect cell line culture. I am having SF9 cells and I want to transfer the culture from one spinner flask to another. I am aware that,this transfer depends on the cell density. I...

11 December 2015 6,949 1 View

How the nanoparticles recognize the tumor cells and specifically kill them??

How the nanoparticles, e.g. metallic nanoparticles or polymer capper particles specifically kill the tumor cells, leaving the healthy and non-cancerous cells unaffected? What is the mechanism by...

08 September 2015 7,389 5 View

How we can surface functionalize the nanoparticles as per our requirement??

How can I engineer the nanoparticles with the desired functional groups at the surface??

08 September 2015 9,942 2 View

How do nanoparticles interact with cancer cells?

08 September 2014 5,259 10 View

How does one determine the concentration of 'drug-loaded nanoparticles'?

I have prepared the drug loaded PLGA nanoparticle. I want to calculate the exact concentration, so that I can carry out further experiments. Please suggest how.

10 November 2013 8,857 11 View

Bio-Nano Interaction.

Primarily I have synthesized silver nanoparticles and checked its anti-colon cancer activity. I just wonder, "how the NPs interact with the cells" and what is its mode of entry into the cells....

07 August 2013 7,802 4 View

Determining the concentration of drug formulated nano-particles?

I have prepared a drug-formulated nanoparticle. I want to check the exact concentration of the NPs, so that I can conduct the designed experiments. The NPs have been coated with PLGA & PVA.

07 August 2013 7,386 29 View

Analysis of Mitochondrial Membrane Potential (MMP) with microscopy.

I want to analyse how a drug is affecting the MMP. So I need to perform the MMP assay with microscopy. I have tried with Mitotracker red dye but have not found promising results. Please suggest...

05 June 2013 3,233 12 View

What are the effective ways to synchronize cells before any cell based experiments?

It is known that people use serum free medium from 2 h to overnight duration for synchronizing cells prior to experiments. My queries are as follows. 1. How serum free medium synchronize the...

01 January 1970 9,520 17 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View